为了对SH2D7 基因序列及其编码蛋白进行生物信息学分析,应用NCBI 数据库和ExPASy 等程序,对SH2D7 基因核苷酸序列进行了分析,并预测了其编码蛋白的理化性质及二级结构等。结果表明,SH2D7 基因定位于人类染色体15q25.1,包含了6 个外显子和5 个内含子。SH2D7 基因编码蛋白的功能主要涉及基因表达调控、信号传导等;分子量约为49.8 kD,等电点为5.99,是一种亲水蛋白,主要位于细胞核中,有35 个磷酸化位点及22 个抗原位点。
The bioinformatic software is used to predict the nucleotide sequence of SH2D7 and the second structure of its encoding protein. It is shown that SH2D7 is located on the human chromosome 15q25.1, containing six exons and five introns. SH2D7 protein is mainly involved in the regulation of the gene expression and the signal transduction. Its molecular weight is 49.8 kD and the isoelectric point is 5.99. SH2D7 is a hydrophilic protein mainly located in the nucleus and there are 35 phosphorylation sites and 22 epitopes bit in the SH2D7 protein sequence.
[1] Orentas R J, Nordlund J, He J, et al. Bioinformatic description of immunotherapy targets for pediatric T-cell leukemia and the impact of normal gene sets used for comparison[J]. Frontiers in Oncology, 2014, 4: 134.
[2] Chu C M, Yao C T, Chang Y T, et al. Gene expression profiling of colorectal tumors and normal mucosa by microarrays meta- analysis using prediction analysis of microarray, artificial neural network, classification, and regression trees[J]. Disease Markers, 2014, 2014: 634123.
[3] Leslie R, O'Donnell C J, Johnson A D. GRASP: Analysis of genotypephenotype results from 1390 genome- wide association studies and corresponding open access database[J]. Bioinformatics, 2014, 30(12): i185-i194.
[4] Park S M, Park S J, Kim H J, et al. A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis[J]. Experimental and Molecular Medicine, 2013, 45: e31.
[5] Sousa J F, Torrieri R, Silva R R, et al. Novel primate-specific genes, RMEL 1, 2 and 3, with highly restricted expression in melanoma, assessed by new data mining tool[J]. PLoS One, 2010, 5(10): e13510.
[6] Laversin S A, Phatak V M, Powe D G, et al. Identification of novel breast cancer-associated transcripts by UniGene database mining and gene expression analysis in normal and malignant cells[J]. Genes Chromosomes Cancer, 2013, 52(3): 316-329.
[7] Aouacheria A, Navratil V, Barthelaix A, et al. Bioinformatic screening of human ESTs for differentially expressedgenes in nomal and tumor tissues[J]. BMC Genomics, 2006, 7: 94.
[8] Barlow D J, Edwards M S, Thornton J M. Continuous and discontinuous protein antigenic determinants[J]. Nature, 1986, 322(6081): 747-748.
[9] Liu W Q, Vidal M, Olszowy C, et al. Structure-activity relationships of small phosphopeptides, inhibitors of Grb2 SH2 domain, and their prodrugs[J]. Journal of Medicinal Chemistry, 2004, 47(5): 1223-1233.
[10] Mandal P K, Ren Z, Chen X, et al. Structure- activity studies of phosphopeptidomimetic prodrugs targeting the Src homology 2 (SH2) domain of signal transducer and activator of transcription 3 (Stat3) [J]. International Journal of Peptide Research and Therapeutics, 2013, 19 (1): 3-12.
[11] Chen K F, Tai W T, Chu P Y, et al. STAT3 mediates regorafenibinduced apoptosis in hepatocellular carcinoma[J]. Clinical Cancer Research, 2014, 20(22): 5768-5776.
[12] Sanz A, Niranjan Y, Hammarén H, et al. The JH2 domain and SH2- JH2 linker regulate JAK2 activity: A detailed kinetic analysis of wild type and V617F mutant kinase domains[J]. Biochimica et Biophysica Acta, 2014, 1844(10): 1835-1841.
