为研究幽门螺杆菌(Helicobacter pylori,Hp)感染对肠道菌群的影响,收集Hp 阴性和Hp 感染(阳性)患者的粪便标本,选择Hp 和肠道菌群中具代表性的大肠杆菌O157、乳酸杆菌、肠球菌、双歧杆菌进行PCR 扩增。实验结果表明,Hp 阴性组中未见Hp 表达,与13C 呼气实验结果基本一致;Hp 阴性组中乳酸杆菌、肠球菌、双歧杆菌表达较高,而Hp 感染组中3 者表达较少;Hp 阴性组中未检测到大肠杆菌O157。幽门螺杆菌感染可改变肠道菌群中乳酸杆菌、肠球菌、双歧杆菌等菌落的数量。
This paper studies the effect of Helicobacter pylori (Hp) infection on gut microbiota using fresh stool specimens collected from normal persons (Hp negative) and patients with Hp infection. Hp, E. coli O157, Lactobacillus, Enterococcus and Bifidobacterium were amplified by PCR. The expression of Hp was not found in Hp negative group, consistent with the 13C breath test. The expression of Lactobacillus, Enterococcus and Bifidobacterium in Hp negative group was higher than that in Hp infection group. There was no E. coli O157 in Hp negative group. The numbers of Lactobacillus, Enterococcus and Bifidobacterium were changed by Hp infection.
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