为研究三磷酸腺苷二钠对慢性高原病(chronic mountain sickness,CMS)大鼠肝脏的保护作用,取60 只SD 大鼠,对其中50 例进行慢性高原病造模后,随机分为高原模型组、硝苯地平对照组、三磷酸腺苷二钠低剂量组、三磷酸腺苷二钠中剂量组、三磷酸腺苷二钠高剂量组,每组10 只;剩余10 只平原环境饲养为正常对照组;造模30 d 后,根据大鼠体重进行干预。连续灌胃15d 后,测定肺动脉压(pulmonary artery pressure,PAP)、同型半胱氨酸(homocysteine,Hcy)、白介素-6(interleukin-6,IL-6)、C-反应蛋白(C-reactionprotein,CRP)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartatetransaminase,AST)等指标。结果发现,与正常对照组比较,高原模型组和硝苯地平对照组大鼠体内的Hcy,IL-6,CRP,MDA,ALT 和AST 水平升高,SOD 和GSH-PX 水平降低,PAP 升高,提示高原低氧对大鼠的肝脏造成损害。与高原模型组比较,三磷酸腺苷二钠高剂量组大鼠体内的Hcy,IL-6,CRP,MDA,ALT 和AST 水平降低(P < 0.05),SOD 和GSH-PX 水平升高(P < 0.05)。故而认为高剂量的三磷酸腺苷二钠对CMS 大鼠肝脏有保护作用。
To study the protective effect of trinosin on the liver of chronic mountain sickness (CMS) rat model, CMS was induced in 50 of 60 SD rats,which were randomly divided into CMS model group, nifedipine control group, high, medium, low dosage trinosin groups and blank control group (10 each). Fifty rats were in low pressure oxygen cabin for 30 days except for the blank control group. After continuous gavage administrationfor 15 days according to their weights,we tested the indexes of pulmonary artery pressure (PAP), homocysteine (Hcy), interleukin- 6 (IL- 6), C- reactionprotein (CRP), superoxide dismutase (SOD), malondialdehyde(MDA), glutathione peroxidase (GSH- PX), alanine aminotransferase (ALT), and aspartate transaminase (AST). The results showed that compared with the blank control group, the CMS model group and nifedipine control group had higher levels of PAP, Hcy, IL-6, CRP, MDA, ALT and AST (P < 0.05), but lower levels of SOD and GSH-PX (P < 0.05), indicating the rat liver was damaged under hypoxic condition. Compared with the CMS model group, the high dosage trinosin group had lower levels ofHcy, IL-6, CRP, MDA, ALT and AST (P < 0.05), but higher levels of SOD and GSH-PX (P < 0.05), indicatinghigh dosage of trinosin had protective effect on the liver of CMS rat model.
[1] 梁远兵, 董现龙. 三磷酸腺苷二钠氯化镁治疗COPD急性加重期患者肺动脉高压疗效观察[J]. 中国临床医生, 2011, 39(8): 54-55. Liang Yuanbing, Dong Xianlong. Clinical observation of adenosine disodium triphosphate and magnesium chloride in the treatment of acute exacerbation of chronic obstructive pulmonary diseases' pulmonary alveolar hypertension[J]. Chinese Journal for Clinicians, 2011, 39(8): 54- 55
[2] 皮凤梅, 屠锡德, 吴悦. 三磷酸腺苷二钠脂质体的制备及其对心肌缺血小鼠组织能量状态的影响[J]. 药学学报, 2010, 45(10): 1322-1326. Pi Fengmei, Tu Xide, Wu Yue. Preparation of ATP-2Na loaded liposome and its effect on tissues energy state in myocardial ischemic mice[J]. Acta Pharmaceutica Sinica, 2010, 45(10): 1322-1326.
[3] 荣黎, 戴立里, 曾维政, 等. 红景天甙对拟高原缺氧大鼠肝损伤的保护作用[J]. 中国组织工程研究与临床康复, 2010, 14(31): 5813-5817. Rong Li, Dai Lili, Zeng Weizheng, et al. Protective effect of salidroside on liver injury of rats under altitude hypoxia simulation[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010, 14(31): 5813- 5817.
[4] Budhiraja R, Tuder R M, Hassoun P M. Endothelial dysfunction in pulmonary hypertension[J]. Circulation, 2004, 109(2): 159-165.
[5] Runo J R, Loyd J E. Primary pulmonary hypertension[J]. Lancet, 2003, 361(9368): 1533-1544.
[6] Flohe S B, Bruggemann J, Herder C, et al. Enhanced Proinflammatory response to endotoxin after priming of maerophages with leadions[J]. Journal of Leukocyte Biology, 2002, 71(3): 417-424.
[7] Hartmann G, Tschop M, Fischer R, et al. High altitude increases circulating interleukin-6, interleukin-1 receptor antagonist and C-reactive protein[J]. Cytokine, 2000, 12(3): 246-252.
[8] De Carvalho J F, Hanaoka B, Szyper-Kravitz M, et al. C-reactive protein and its implications in systemic lupus erythematosus[J]. Acta Reumatologia Port, 2007, 32(4): 317-322.
[9] Olsson M L, Clausen H. Modifying the red cell surface: Towards an ABOuniversal blood supply[J]. British Journal of Haematology, 2008, 140(1): 3-12.
[10] 王灿, 苗志敏, 李长贵, 等. 人体血尿酸水平对血清谷丙转氨酶和谷草转氨酶水平的影响[J]. 山东医药, 2010, 50(29): 1-3. Wang Can, Miao Zhimin, Li Changgui, et al. Impact of uric acid on alanine amiotransferase and aspartate aminotransferase[J]. Shandong Medical Journal, 2010, 50(29): 1-3.
[11] 高志, 邓友章. 中药骨筋宝汤对膝关节骨性关节炎患者血清SOD、NO 及MDA水平的影响[J]. 海南医学院学报, 2012, 18(3): 341-343. Gao Zhi, Deng Youzhang. Influence of Gujing Bao Tang on SOD, NO and MDA levels in patients osteoarhritis of the knee[J]. Journal of Hainan Medical University, 2012, 18(3): 341-343.
[12] 李洁, 张耀斌, 邢良美. 不同低氧训练模式对大鼠肝脏及肾脏组织内自由基代谢的影响[J]. 体育科学, 2008, 28(5): 56-61. Li Jie, Zhang Yaobin, Xing Liangmei. Effect of different hypoxic training methods on free radical metabolism of liver and kidney tissue in mice[J]. China Sport Science, 2008, 28(5): 56-61.
[13] 秦德安, 苏丹, 王晓玲. 橙皮苷对羟自由基的消除作用[J]. 中国药学杂志, 1996, 31(7): 396-398. Qin Dean, Su Dan, Wang Xiaoling. Scavenging action to hydroxyl free radical of hesperidin[J]. Chinese Pharmaceutical Journal, 1996, 31(7): 396-398.
[14] 崔建华, 张西洲, 王宏运, 等. 慢性高原病肝功能及血清酶的改变[J]. 临床军医杂志, 2006, 34(6): 677-678. Cui Jianhua, Zhang Xizhou, Wang Hongyun, et al. Liver function and serum enzyme changes of chronic mountain in sickness[J]. Clinical Journal of Medical Officer, 2006, 34(6): 677-678.
[15] MartinR,FitzlG,MozetC,etal. Effectofageandhypoxia/reoxygenation on mRNA expression of antioxidative enzymes in rat liver and kidneys[J]. Experimental Gerontology, 2002, 37(12): 1481-1487.
