科技导报 >

2015 , Vol. 33 >Issue 11: 84 - 89

DOI: https://doi.org/10.3981/j.issn.1000-7857.2015.11.015

研究论文

异常黑胆质成熟剂对异常黑胆质型肝癌病证大鼠模型肝脏形态学的影响

  • 王延蛟, 热沙来提·阿不都瓦衣特, 哈木拉提·吾甫尔, 热斯拉特·艾力木, 阿尤甫江·阿布都热依木, 斯坎德尔·白克力
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  • 1. 新疆医科大学基础医学院, 乌鲁木齐830011;
    2. 新疆医科大学第一附属医院, 乌鲁木齐830054;
    3. 新疆医科大学维吾尔医学院, 乌鲁木齐830011
王延蛟,副教授,研究方向为维医异常黑胆质型肝癌发病分子机制,电子信箱:dss-02458@163.com

收稿日期: 2015-02-09

  修回日期: 2015-03-26

  网络出版日期: 2015-06-11

基金资助

国家自然科学基金项目(81160545)

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The effect of abnormal savda munziq on hepatocarcinoma cell morphology of abnormal savda rat

  • WANG Yanjiao, ABDUWAYIT Resalat, UPUR Halmurat, HELIM Resalat, ABDUREYIM Ayupjan, BAKRI Iskandar
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  • 1. College of Basic Medical, Xinjiang Medical University, Urumqi 830011, China;
    2. The First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, China;
    3. College of Uygur Medicine, Xinjiang Medical University, Urumqi 830011, China

Received date: 2015-02-09

  Revised date: 2015-03-26

  Online published: 2015-06-11

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摘要

为探讨异常黑胆质成熟剂对异常黑胆质型肝癌病证大鼠模型肝脏形态学的影响, 按照维吾尔医模型建立标准, 采用干寒环境、干寒饲料和异常物理刺激等多重因素复合作用, 建立维医异常黑胆质证载体动物模型, 在此基础上, 通过饮用灭菌食用水配制浓度为0.1 mg/mL 二乙基亚硝胺(DEN)溶液建立异常黑胆质型肝癌病证载体大鼠模型, 并用大、中、小不同剂量(6.0、3.0、1.5 g/kg)异常黑胆质成熟剂进行全程干预, 共分为病证模型组、小剂量组、中剂量组、大剂量组、正常对照组、肝癌对照组6 组, 每组24 只。干预20 周时处死大鼠, 对肝脏的病理变化和超微结构进行观察。研究发现, 20 周时, 低剂量组与病证模型组相比, 形态无明显差异, 高剂量组和中剂量组与病证模型组相比, 具有肝细胞结缔组织增生减少、间质炎性细胞浸润程度明显减轻、肝细胞水肿程度减轻等病理表现及肝细胞内细胞器分布不均匀、肝糖原和线粒体逐渐减少等超微结构明显变化。研究表明, 异常黑胆质成熟剂对异常黑胆质型肝癌病证模型的肝脏组织形态改变有一定的保护和修复作用。

关键词： 维吾尔医学; 异常黑胆质成熟剂; 形态学; 肝癌

本文引用格式

王延蛟, 热沙来提·阿不都瓦衣特, 哈木拉提·吾甫尔, 热斯拉特·艾力木, 阿尤甫江·阿布都热依木, 斯坎德尔·白克力 . 异常黑胆质成熟剂对异常黑胆质型肝癌病证大鼠模型肝脏形态学的影响[J]. 科技导报, 2015 , 33(11) : 84 -89 . DOI: 10.3981/j.issn.1000-7857.2015.11.015

Abstract

To find how the abnormal savda munziq affects the hepatocarcinoma cell morphology of abnormal savda rat, rats were under the stimulations of dry cold environment and dry cold feed and abnormal physical stimulation to establish a model with the abnormal savda according to Uyghur medicine theory. They were raised by sterilized edible water with 0.1 mg/mL of diethylnitrosamine (DEN) solution to establish the hepatocarcinoma model. The rats were treated with high and middle and low doses (6.0, 3.0, 1.5 g/kg) in three groups during the whole period. There were the abnormal savda and HCC model group, low dosage group, middle dosage group, high dosage group, normal control group, and HCC control group, with 24 rats in each group. The animals were anesthetized and killed at the 20th week, then HE staining and observation of morphological characteristics of livers were performed. The results revealed the followings: Albeit no significant differences were found between low dosage group and model groups in morphology, compared with the model groups in high dosage or middle dosage, hyperplasia of connective tissue decreases and the degree of interstitial infiltration of inflammatory cells reduced significantly, cell edema alleviated, reductions of organelle nonuniform, glycogen and mitochondria were significant in the model group. Our findings indicate that the abnormal savda munziq can depress the transformation of hepatocyte to hepatocellular carcinoma in the process of chimeric rats.

Key words： Uyghur medicine; abnormal savda munziq; morphology; hepatocarcinoma

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