为探讨吉林人参低聚肽对实验小鼠的抗疲劳作用,将336 只SPF 级雄性ICR 小鼠随机分为7 组,分别为空白对照组、乳清蛋白组(0.500 g/kg)及5 个吉林人参低聚肽剂量组(0.125、0.250、0.500、1.000、2.000 g/kg).连续灌胃30 d 后,进行负重游泳实验测定各组小鼠负重游泳力竭时间,采用全自动生化仪测定各组小鼠血清尿素氮含量和乳酸脱氢酶活力,采用紫外分光光度计法检测各组小鼠血乳酸水平,采用试剂盒检测各组小鼠肝糖原含量.各项指标中空白对照组与乳清蛋白组之间均无统计学差异;与乳清蛋白组相比,适当剂量的吉林人参低聚肽干预可提高小鼠运动耐力,延长负重游泳时间,降低运动后血乳酸和血尿素氮的含量,提高乳酸脱氢酶活性和肝糖原的含量.因此,吉林人参低聚肽具有缓解实验小鼠体力疲劳功能.
The present study investigated the anti-fatigue effects of Panax ginseng oligopeptide of Jilin. 336 male ICR mice of SPF were randomized divided into 7 groups: control group, whey protein group, and other five groups of panax ginseng oligopeptide of Jilin at doses of 0.125, 0.250, 0.500, 1.000, 2.000 g/kg, respectively. After being fed for 30 days, the loading swimming time was determined by swimming experiment. The content of blood urea nitrogen and the activity of lactic dehydrogenase were measured by an automatic biochemical analyzer. The level of blood lacticacid in mouce was detected by ultraviolet spectrophotometer, and the liver glycogen of mouce was measured according to the kit. There were no significant differences between the control group and whey protein group in terms of the above indicators. Compared with the whey protein group, Panax ginseng oligopeptide of Jilin could significantly increase the burden swimming time, decrease the content of blood urea nitrogen and lactic acid, improve the activity of lactic dehydrogenase and increase the content of liver glycogen in mouce. Thus, it is concluded that panax ginseng oligopeptide of Jilin has excellent anti-fatigue effects in mouce.
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