探讨海藻玉壶汤加减海藻甘草反药组合对大鼠甲状腺肿大的治疗作用。实验将Wistar 大鼠随机分为7 组,空白组灌服生理盐水,其余各组连续14 d灌服丙硫氧嘧啶复制甲状腺肿大模型,以优甲乐作为阳性对照药,各组分别给予相应治疗药物,1次/d,连续28 d。测定甲状腺系数、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)、游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)、促甲状腺激素(TSH)、促甲状腺激素释放激素(TRH)水平,采用苏木精-伊红染色法(HE 染色)观察各组大鼠甲状腺组织形态。结果显示,海藻玉壶汤全方组、去海藻组、去甘草组以及去海藻甘草组的甲状腺系数较模型组明显降低,具有统计学差异(P<0.05);给药各组T3、T4水平较模型组有升高的趋势,但无统计学差异;给药各组对TSH 的水平有明显降低,具有统计学差异(P<0.05);全方组的TRH 水平较模型组明显降低,具有统计学差异(P<0.05),去海藻组、去甘草组和去海藻甘草组有降低的趋势,但无统计学差异。HE 染色镜下结果显示,给药各组对甲状腺肿大均有恢复作用,全方组效果最为显著。根据检测结果,优选配比条件下,含反药配伍的海藻玉壶汤全方组对甲状腺肿大的恢复作用以及甲状腺各项指标回调效果优于全方去单味海藻、甘草或同时去掉海藻甘草的配伍组合。
To explore the therapeutic effect of HaizaoYuhu decoction with or without seaweed and liquorice anti-drug combination on goiter rats, Wistar rats were randomly divided into 7 groups. The blank group were gavaged normal saline, the other groups were fed propylthiouracil copy goiter model,with the Euthyrox as a positive control drug. Continuous administration of drugs was used once a day for 28 days To detect the thyroid coefficients, T3, T4, FT3, FT4, TSH and TRH, the thyroid tissue morphology in each group was observed by HE staining. The result showed that the thyroid coefficients of each group are lower than those of the model group and have a significant difference (P<0.05). Compared with the model group, T3 and T4 of each group have a trend of increase but no significant difference. TSH of each group decreases obviously and has a significant difference (P<0.05). Compared with the model group, TRH of full formula group decreases obviously and has a significant difference (P<0.05), full formula group without seaweed, full formula group without liquorice, full formula group without seaweed and liquorice have a trend of decrease but no significant difference. According to the result, Haizao Yuhu decoction full formula group can better recover goiter and callback indicators in preferred compatibility proportion conditions than full formula group without seaweed, full formula group without liquorice, and full formula group without seaweed and liquorice.
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