In order to standardize the syndrome of traditional Uyghur medicine, abnormal Savda rat model with asthma is selected as study subject and metabolic changes are investigated in animal model and clinical patient to reveal the similarity and difference between them. Sprague Dawley (SD) rats are subjected to abnormal Savda-producing conditions, followed by induction of asthma. Clinical patients of abnormal Savda with asthma are also selected as study subjects. Serum samples are collected from each test group and analyzed by nuclear magnetic resonance (NMR). Spectral profiles are analyzed by multivariate analysis using orthogonal projection to latent structure with discriminant analysis (OPLS-DA). Results show that compared with healthy controls, leucine, isoleucine, valine, alanine, lipid related metabolites like VLDL, and unsaturated lipids concentration in plasma of patients are decreased, but acetic acid, acetone, acetoacetate and carnitine are significantly increased; glutamate in patients also shows a significant increase while creatine, creatinine, scyllo-inositol and lactate are significantly reduced. For animal models, the change in concentration of lipid related metabolites and lactate, carnitine and glutamine is consistent with the clinical patient, but the amino acid displays an opposite trend. Alanine and creatinine concentrations are higher in plasma of abnormal Savda rat model compared to abnormal Savda with asthma rat model. It is concluded that although the amino acid metabolism in rat model is different from that in clinical patient, their changes of lipid related metabolites contents are highly consistent. Fat related metabolites may be considered biomarkers of abnormal Savda.
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