两性霉素B是多烯类广谱抗真菌抗生素，已广泛应用于深部真菌感染。以结节链霉菌Streptomyces nodosus ZJB15076为出发菌种，对其发酵生产两性霉素B的培养基成分进行了优化，确定了较优的碳源和氮源及最适浓度：质量分数为葡萄糖4.5%、牛肉膏4%；在此基础上继续进行发酵条件的研究，确定了较优的发酵条件：发酵初始pH值为7.0，培养温度为28℃，装液量为40 mL/250 mL，接种量（体积分数）为2%。分析了在摇瓶内添加玻璃珠对两性霉素B产量的影响，研究结果表明，添加玻璃珠后能显著改善菌丝聚集情况并显著提高两性霉素B的产量，产量与对照组相比提高了341.1%，达到4563.2 mg/L。
Amphotericin B(AmB) is one of the most efficient antimycotic agents, thus widely used in clinical practice. In this study, Streptomyces nodosus ZJB15076 is used for AmB fermentation. The optimal carbon source, nitrogen source and fermentation conditions for the production of AmB are tested. The results show that glucose and nitrogen beef extraction is best for the fermentation of AmB. And the optimized fermenting conditions through single factor test are summed up as follows:culture temperature 28℃, initial pH value 7.0, inoculation 2% and liquid volume 40 mL/250 mL. The influence of adding glass bead is significant. After adding glass bead, the production of AmB is increased by 341.1% and reaches 4563.2 mg/L.
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