The protective effect of Angelica keiskei chalcones on the damage of HaCaT cells induced by ultraviolet B(UVB) is investigated.. HaCaT cell with oxidative damage induced by ultraviolet B is incubated with Angelica keiskei chalcones at concentrations of 20 μmol/L, 10 μmol/L and 5 μmol/L for 24 h. The cell viability as well as the apoptosis rate are determined by MTT method and flow cytometer, respectively. The MDA content of cells and activities of SOD, CAT and Caspase-3 are determined by kit method. The mRNA expression of BCL-2 and Bax is evaluated by reverse transcription polymerase chain reaction (RT-PCR). Compared with the normal control, the cell viability, SOD, CAT activity and the expression level of BCL-2 mRNA in the irradiation model group are decreased while the MDA content, apoptosis rate, Caspase-3 activity and Bax mRNA are increased. The viability, SOD, CAT activity and BCL-2 mRNA expression of cells incubated with 20 μmol/L and 10 μmol/L are increased while the apoptosis rate, Caspase-3 activity and Bax mRNA expression are decreased, compared with the irradiation model group. All the differences are significant (P<0.05). Angelica keiskei chalcones may protect HaCaT cells from oxidative damage and decrease apoptosis rate by regulating the mRNA expression of BCL-2 and Bax.
[1] 步犁, 程树军, 谈伟君, 等. 人角质细胞紫外线损伤模型筛选抗氧化剂的研究[J]. 毒理学杂志, 2013, 27(6):419-423. Bu Li, Cheng Shujun, Tan Weijun, et al. The screening study of antioxidant to UV-induced HaCaT model[J]. Journal of Toxicol-ogy, 2013, 27(6):419-423.
[2] Greinert R B, Henning V S. UVA-induced DNA doublestrand breaks result from the repair of clustered oxidative DNA damage[J]. Nucleic Acids Research, 2012, 40(20):10263-10273.
[3] 胡青梅, 景海霞, 雷铁池. UVA及UVB诱导人皮肤光生物学反应差异的研究进展[J]. 临床与病理杂志, 2017, 37(1):199-202. Hu Qingmei, Jing Haixia, Lei Tiechi. Progress on difference of photobiology reaction about UVA and UVB radiation on human skin[J]. Journal of Clinical and Pathological Research, 2017, 37(1):199-202.
[4] Choi Y H, Lee H S, Chung C K, et al. Protective effects of an ethanol extract of Angelica keiskei against acetaminophen-induced hepatotoxicity in HepG2 and HepaRG cells[J]. Nutrition Research And Practice, 2017, 11(2):97-104.
[5] Kusuma S A F, Iskandar Y, Dewanti M A. The ethanolic extract of ashitaba stem (Angelica keskei) as future antituberculosis[J]. Journal of Advanced Pharmaceutical Technology & Research, 2018, 9(1):37-41.
[6] 王先红, 张照杰, 李蕾, 等. 明日叶查尔酮对糖尿病大鼠氧化应激影响[J]. 中国公共卫生, 2014, 30(1):74-76. Wang Xianhong, Zhang Zhaojie, Li Lei, et al. Effect of ashitaba chalcone on oxidative stress in diabetic rats[J]. Chinese Journal of Public Health, 2014, 30(1):74-76.
[7] Tabata K, Motani K, Takayanagi N, et al. Xanthoangelol, a major chalcone constitute of Angleica keiskei, induces apoptosis in neuroblastoma and leukemia cells[J]. Biological and Pharmaceutical Bulletin, 2005, 28(8):1404-1407.
[8] Enoki T, Ohnoqi H, Nagamine K, et al. Antidiabetic activities of chalcones isolated from a Japanese Herb Angelica keiskei[J]. Journal of Agricultural and Food Chemistry, 2007, 55(15):6013-6017.
[9] Fuso A, Nicolia V, Cavallaro R A, et al. DNA methylase and demethylase activities are modulated by one-carbon metabolism in Alzheimer's disease models[J]. Journal of Nutritional Biochemistry, 2011, 22(3):242-251.
[10] Obeid R, Schadt A, Dillmann U, et al. Methylation status and neurodegenerative markers in Parkinson disease[J]. Clinical Chemistry, 2009, 55(10):1852-1860.
[11] 翟苗苗, 刘玉娟, 刘庆平, 等. 明日叶查尔酮对小鼠急性辐射损伤防护作用的研究[J]. 毒理学杂志, 2015, 29(3):204-206. Zhai Miaomiao, Liu Yujuan, Liu Qingping, et al. Research on protective effect of Ashitaba Chalcone on acute radiationinjured mice[J]. Journal of Toxicology, 2015, 29(3):204-206.
[12] 刘硕, 邝梦婷, 朱华伟, 等. 蔓越莓抑制UVB诱导HaCaT细胞氧化损伤和凋亡的研究[J]. 食品研究与开发, 2015, 369(11):5-10. Liu Shuo, Kuang Mengting, Zhu Huawei, et al. Study on the inhibition effects of cranberry on UVB-induced oxidative damage and apoptosis of HaCaT cells[J]. Food Research and Development, 2015, 369(11):5-10.
[13] 邓蕙妍, 高爱莉, 张倩雯, 等. 姜黄素对紫外线诱导损伤的HaCaT细胞的保护作用[J]. 中国中西医结合皮肤性病学杂志, 2014, 13(3):137-141. Deng Huiyan, Gao Aili, Zhang Qianwen, et al. Protective effect of curcumin on human immortalized keratinocytes after acute ultraviolet damage[J]. Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine, 2014, 13(3):137-141.
[14] 马蕊, 刘仲华, 黄建安, 等. 绿茶和红茶提取物抑制中波紫外线诱导HaCaT细胞氧化损伤和凋亡的比较[J]. 湖南农业大学学报(自然科学版), 2013, 39(4):377-381. Ma Rui, Liu Zhonghua, Huang Jianan, et al. Comparation the inhibitional effects between extracts of green tea and black tea on oxidative damage and apoptosis of HaCaT cells induced by UVB[J]. Journal of Hunan Agricultural University (Natural Sciences), 2013, 39(4):377-381.
[15] 高扬, 易若琨, 宋家乐. 竹叶总黄酮对UVB诱导HaCaT细胞氧化损伤的保护作用[J]. 南京中医药大学学报, 2015, 31(2):165-169. Gao Yang, Yi Ruokun, Song Jiale. Protective effects of total flavonoids from bamboo leaves on UVB-irradiated oxidative damage in HaCaT cells[J]. Journal of Nanjing University of Chinese Medicine, 2015, 31(2):165-169.
[16] 侯芳霖, 钟进义, 张燕. 明日叶查尔酮对荷瘤小鼠抗氧化能力影响作用的研究[J]. 世界中西医结合杂志, 2011, 6(4):288-290. Hou Fanglin, Zhong Jinyi, Zhang Yan. Research of Ashitaba Chalcone on the antioxidant effect in tumor-bearing mice[J]. World Journal of Integrated Traditional and Western Medicine, 2011, 6(4):288-290.
[17] 李金莲, 严州萍, 陈雪红, 等. 扇贝多肽抑制紫外线A波诱导的HaCaT细胞凋亡依赖p38 MAPK通路和caspase-3[J]. 中国药学杂志, 2007, 42(2):116-120. Li Jinlian, Yan Zhouping, Chen Xuehong, et al. Inhibition of Polypeptide from chlamys farreri on UVA-Induced apoptosis of HaCaT cells depending on p38 MAPK pathway and caspase-3[J]. Chinese Pharmaceutical Journal, 2007, 42(2):116-120.
[18] Hockenbery D M, Nunez G, Milliman C, et al. BCL-2 is an inner mitochondrial membrane protein that blocks programmed cell death[J]. Nature, 1990, 348(6299):334-338.
[19] Adams J M, Cory S. The BCL-2 arbiters of apoptosis and their growing role as cancer targets[J]. Cell Death and Differentiation, 2018, 25(1):27-36.
