专题:新污染物治理

苯并[a]芘厌氧降解菌群的富集及其条件优化

  • 易倩文 ,
  • 肖芳 ,
  • 邹晓爽 ,
  • 李江 ,
  • 张云涛 ,
  • 代永恒 ,
  • 王斌 ,
  • 侯立安
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  • 1. 贵州大学资源与环境工程学院, 喀斯特地质资源与环境教育部重点实验室(贵州大学), 贵阳 550025;
    2. 贵州喀斯特环境生态系统教育部野外科学观测研究站, 贵阳 550025;
    3. 贵州大学土木工程学院, 贵阳 550025;
    4. 中国人民解放军96901部队23分队, 北京 100094
易倩文,硕士研究生,研究方向为环境生物技术,电子信箱:qwy199807@163.com;李江(通信作者),教授,研究方向为有机固废资源化和新污染物治理,电子信箱:jli82@gzu.edu.cn;侯立安(共同通信作者),中国工程院院士,正高级工程师,研究方向为饮用水安全保障、分散点源生活污水处理和人居环境空气净化等,电子信箱:h20091957@126.com

收稿日期: 2024-01-02

  修回日期: 2024-04-28

  网络出版日期: 2024-07-08

基金资助

国家自然科学基金项目(5196808);贵州大学科研创新团队项目(贵大科创团[2023]05号)

Enrichment of benzopyrene anaerobic degrading bacteria and optimization of its conditions

  • YI Qianwen ,
  • XIAO Fang ,
  • ZOU Xiaoshuang ,
  • LI Jiang ,
  • ZHANG Yuntao ,
  • DAI Yongheng ,
  • WANG Bin ,
  • HOU Li'an
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  • 1. Key Laboratory of Karst Georesources and Environment Ministry of Education, College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025, China;
    2. Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang 550025, China;
    3. College of Civil Engineering, Guizhou University, Guiyang 550025, China;
    4. Detachment 23, Unit 96901 People's Liberation Army of China, Beijing 100094, China

Received date: 2024-01-02

  Revised date: 2024-04-28

  Online published: 2024-07-08

摘要

从污泥-秸秆联合厌氧污泥体系中富集出以 BaP 为唯一碳源的菌群——YQ-BaP 菌群,通过单因素实验研究了不同环境因素对菌群降解能力的影响,采用 GC-MS 分析苯并[a]芘降解的中间代谢产物。结果表明:该菌群优势菌属为Pseudomonas和Methyloversatilis。该菌群在培养 14 d 后对 30 mg/L BaP 的降解率稳定在 38.69%±6.24%,对菲和芘的降解效果更佳。YQ-BaP 菌群在 pH=7和 35~40℃时表现出较强的降解能力;当 BaP 初始浓度为 20 mg/L时,对 BaP降解率达到 46.89%。GC-MS分析表明,BaP在降解过程中产生菲、蒽和萘等羟化和甲基化代谢产物。研究显示,该菌群具有较强的BaP降解能力,可为多环芳烃污染环境的生物修复提供理论基础和技术支撑。

本文引用格式

易倩文 , 肖芳 , 邹晓爽 , 李江 , 张云涛 , 代永恒 , 王斌 , 侯立安 . 苯并[a]芘厌氧降解菌群的富集及其条件优化[J]. 科技导报, 2024 , 42(11) : 47 -57 . DOI: 10.3981/j.issn.1000-7857.2024.01.00017

Abstract

The YQ-BaP microbial community with Benzo[a]pyrene (BaP) as the sole carbon source was enriched from the sludge straw combined anaerobic sludge system. The effects of different environmental factors on the biodegradability of bacteria were studied by single factor experiment. The intermediate metabolites degraded by benzo[a]pyrene were analyzed by gas chromatography-mass spectrometry (GC-MS). The results of macrogene sequencing showed that the dominant genera of this bacterium were Pseudomonas and Methyloversatilis. After 14 days of culture, the degradation rate of 30 mg/L BaP was stable at 38.69%±6.24%, and the degradation effect of phenanthrene and pyrene was better. YQ-BaP showed strong degradation ability at pH=7 and 35~40℃. When the concentration of BaP was 20 mg/L, the degradation rate of BaP reached 46.89%. GC-MS analysis showed that phenanthrene, anthracene, naphthalene and other hydroxylation and methylation metabolites were produced during BaP degradation. The bacterial community has a strong ability to degrade BaP, which provides a theoretical basis for the remediation technology of PAHs pollution.

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