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柑橘黄龙病检测及防控方法研究进展

  • 张天宇 ,
  • 杨钙仁 ,
  • 何寻阳 ,
  • 刘秋梅 ,
  • 李德军
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  • 1. 广西大学林学院, 南宁 530004;
    2. 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室, 长沙 410125;
    3. 河池环江农田生态系统广西野外科学观测研究站, 环江 547100
张天宇,硕士研究生,研究方向为黄龙病的微生物防控,电子信箱:1749012933@qq.com;杨钙仁(通信作者),教授,研究方向为生态农业等,电子信箱:yanggr@gxu.edu.cn;何寻阳(共同通信作者),研究员,研究方向为土壤微生物生态等,电子邮箱:hbh-pjhn@isa.ac.cn

收稿日期: 2023-09-25

  修回日期: 2024-02-22

  网络出版日期: 2024-07-08

基金资助

广西重点研发计划项目(桂科AB22080097)

Research progress on detection and control method of citrus Huanglongbing

  • ZHANG Tianyu ,
  • YANG Gairen ,
  • HE Xunyang ,
  • LIU Qiumei ,
  • LI Dejun
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  • 1. College of Forestry, Guangxi University, Nanning 530004, China;
    2. Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    3. Hechi Huanjiang Agriculture Ecosystem Field Scientific Observation and Research Station of Guangxi, Huangjiang 547100, China

Received date: 2023-09-25

  Revised date: 2024-02-22

  Online published: 2024-07-08

摘要

柑橘黄龙病是在柑橘产业中最具危害的细菌传染性病害,是柑橘产业健康长久发展的重大威胁。综述了柑橘黄龙病病原菌及其致病机理的研究进展,分析了以纳米孔测序和图像数据集为主的快速检测柑橘黄龙病的方法,梳理了目前防控柑橘黄龙病的主要手段,包括传统防控方法、抗病育种、药物制剂防控和生物防控等。结合目前柑橘黄龙病抗(耐)病性与防控、检测技术的基本现状,提出可在建立黄龙病菌体外培养技术、发展田间快速检测技术、抗(耐)病新品种的规模化产出创新技术、柑橘黄龙病菌致病机理和绿色防控等方向深入研究。

本文引用格式

张天宇 , 杨钙仁 , 何寻阳 , 刘秋梅 , 李德军 . 柑橘黄龙病检测及防控方法研究进展[J]. 科技导报, 2024 , 42(11) : 75 -83 . DOI: 10.3981/j.issn.1000-7857.2023.09.01425

Abstract

Citrus Huanglongbing is the most destructive bacterial infectious disease in the citrus industry, posing a significant threat to the healthy and long-term development of the citrus industry. This article elaborates on the research progress of the pathogens and pathogenic mechanisms of citrus Huanglongbing, analyzes rapid detection methods of citrus Huanglongbing which are mainly based on nanopore sequencing and image datasets, and summarizes the current main methods for preventing and controlling citrus Huanglongbing, including traditional prevention and control methods, disease resistant breeding, drug formulation prevention and control, and biological prevention and control. Based on the current basic status of resistance, prevention, and detection technologies for citrus Huanglongbing, it is proposed to conduct in-depth research in establishment of in vitro cultivation technology for Candidatus Liberibacter asiaticus(CLas), development of rapid field detection technology, innovative technologies for large-scale production of new resistant (tolerant) varieties, pathogenic mechanisms of citrus Huanglongbing, and green prevention and control.

参考文献

[1] 张旭颖,岑伊静.亚洲柑橘木虱与柑橘黄龙病菌互作的研究进展[J].环境昆虫学报, 2020, 42(3):630-637.
[2] 唐利华,郭堂勋,李其利,等.柑橘黄龙病田间诊断与检测技术研究进展[J].中国植保导刊, 2018, 38(8):81-87.
[3] 黄峰,崔一平,宋晓兵,等.黄龙病对柑橘叶际微生物组的影响[J].植物保护学报, 2023, 50(5):1150-1160.
[4] 黄家权,李莉,吴丰年,等.携带不同原噬菌体的黄龙病菌在柑橘木虱体内的增殖及致病力[J].中国农业科学, 2022, 55(4):719-728.
[5] 白华菊,龙梦玲.柑橘黄龙病研究进展[J].中国植保导刊, 2009, 29(3):13-16.
[6] 陈文利,徐婉,程保平,等.柑橘黄龙病检测及治疗方法的研究进展[J].华南师范大学学报(自然科学版), 2017, 49(5):9-15.
[7] 秦紫芳,石旺鹏.木虱寄生蜂研发现状及趋势[J].中国生物防治学报, 2021, 37(5):1082-1089.
[8] 黎海霖,郑霞林,王小云,等.柑橘木虱成虫繁殖行为前期及活动规律研究[J].南方农业学报, 2019, 50(9):2009-2014.
[9] 程春振,曾继吾,钟云,等.柑橘黄龙病研究进展[J].园艺学报, 2013, 40(9):1656-1668.
[10] 乌天宇,张旭颖, George A C B,等.亚洲柑橘木虱成虫和5龄若虫在感染黄龙病的柑橘上的取食行为及获菌效率比较[J].昆虫学报, 2020, 63(2):166-173.
[11] Fan J, Chen C, Brlansky R H, et al. Changes in carbohy-drate metabolism in citrus sinensis infected with "Candi-datus Liberibacter asiaticus" [J]. Plant Pathology, 2010(59):1037-1043.
[12] Texeira D C, Ayres J, Kitajima E W, et al. First report of a huanglongbing-like disease of Citrus in Sao Paulo state, Brazil and association of a new Liberibacter spe-cies, "Candidatus Liberibacter americanus", with the dis-ease[J]. Plant Disease, 2005, 89(1):107.
[13] 谢攀.柑橘黄龙病病原菌共培养体系的构建[D].重庆:重庆大学, 2015.
[14] 樊晶.柑橘宿主对黄龙病病原菌侵染的应答机制[D].重庆:重庆大学, 2010.
[15] 吴英林.柑橘黄龙病疫情监测与防控措施[J].现代农业科技, 2022(4):117-118, 125.
[16] 文庆利,谢竹,吴柳,等.柑橘响应黄龙病侵染的韧皮部蛋白2基因CsPP2B15的克隆与表达分析[J].园艺学报, 2018, 45(12):2347-2357.
[17] Ma W X, Pang Z Q, Huang X E, et al. Citrus huanglong-bing is a pathogen-triggered immune disease that can be mitigated with antioxidants and gibberellin[J]. Nature Communications, 2022, 13:529.
[18] 王许会,郭洋洋,全金成,等.柑橘黄龙病碘-淀粉法快速检测技术优化[J].福建农林大学学报(自然科学版), 2017, 46(4):392-396.
[19] 晏建红,关巍,宾羽,等.柑橘黄龙病菌分泌蛋白05150的筛选、原核表达及抗血清制备[J].果树学报, 2020, 37(9):1384-1393.
[20] 黄伟锋,洪添胜,吴伟斌,等.柑橘黄龙病检测方法研究进展[J].广东农业科学, 2012, 39(16):60-64, 239.
[21] 李彬,罗聪,苏玲,等.柑橘黄龙病病原快速检测技术研究进展[J].基因组学与应用生物学, 2020, 39(2):652-657.
[22] Keremane M L, McCollum T G, Roose M L, et al. An improved reference gene for detection of "Candidatus Li-beribacter asiaticus" associated with Citrus huanglong-bing by qPCR and digital droplet PCR assays[J]. Plants, 2021, 10(10):2111.
[23] Hu T S, Chitnis N, Monos D, et al. Next-generation se-quencing technologies:An overview[J]. Human Immunol-ogy, 2021, 82(11):801-811.
[24] 卢慧林,陈大嵩,陈逢浩,等.基于纳米孔测序技术的柑橘黄龙病检测方法建立[J].环境昆虫学报, 2021, 43(6):1596-1600.
[25] Wang Y H, Zhao Y, Bollas A, et al. Nanopore sequenc-ing technology, bioinformatics and applications[J]. Na-ture Biotechnology, 2021, 39(11):1348-1365.
[26] 路惠馨,孙凯,尹传林,等.纳米孔测序技术在植物病原检测中的应用与展望[J].农业生物技术学报, 2021, 29(9):1817-1824.
[27] 李中浤,杜彩丽,林彦锋,等.纳米孔测序技术在环境微生物研究中的应用[J].生物工程学报, 2022, 38(1):5-13.
[28] 范世达,马伟荣,姜文博,等.基于深度学习的柑橘黄龙病远程诊断技术初探[J].中国果树, 2022(4):76-79.
[29] 曾伟辉,陈亚飞,胡根生,等. SMS和双向特征融合的自然背景柑橘黄龙病检测技术[J].农业机械学报, 2022, 53(11):280-287.
[30] He C C, Li X B, Liu Y S, et al. Combining multicolor fluorescence imaging with multispectral reflectance imag-ing for rapid Citrus huanglongbing detection based on lightweight convolutional neural network using a hand-held device[J]. Computers and Electronics in Agricul-ture, 2022, 194:106808.
[31] 林少丹,李效彬,杨碧云,等.适用于小样本显微图像数据集的柑橘黄龙病快速诊断模型[J].农业工程学报, 2022, 38(12):216-223.
[32] Gómez-Flores W, Garza-Saldaña J J, Varela-Fuentes S E. Detection of huanglongbing disease based on intensi-ty-invariant texture analysis of images in the visible spectrum[J]. Computers and Electronics in Agriculture, 2019, 162:825-835.
[33] Liu Y D, Xiao H C, Xu H, et al. Visual discrimination of citrus HLB based on image features[J]. Vibrational Spectroscopy, 2019, 102:103-111.
[34] Su C, Ding F, Wang W J, et al. Time-resolved fluores-cent microsphere lateral flow biosensors for rapid detec-tion of Candidatus Liberibacter asiaticus[J]. Plant Bio-technology Journal, 2022, 20(7):1235-1237.
[35] Puspitasari, Yunimar, Sastro Y. Utilization of rapid de-tection kit in measuring huanglongbing outbreak level:Case study in Koto Tinggi, West Sumatera, Indonesia[J]. IOP Conference Series Earth and Environmental Sci-ence, 2020, 468(1):012046.
[36] Weng J F, Zhang J Z, Zhang C Y, et al. Effective detec-tion of early Citrus huanglongbing by polyethyleneimine modified multi-walled carbon nanotubes gas sensor[J]. Sensors and Actuators B-Chemical, 2022, 371:132508.
[37] 周常勇.对柑橘黄龙病防控对策的再思考[J].植物保护, 2018, 44(5):30-33.
[38] 胡雪芳,田志清.柑橘黄龙病防治技术研究进展[J].中国植保导刊, 2021, 41(7):32-38, 20.
[39] 王飞燕,张瑞敏,吴文,等.柑橘黄龙病对沙田柚树体特性和果实品质的影响[J].热带作物学报, 2020, 41(9):1847-1855.
[40] Zou X P, Jiang X Y, Xu L Z, et al. Transgenic Citrus ex-pressing synthesized cecropin B genes in the phloem ex-hibits decreased susceptibility to huanglongbing[J]. Plant Molecular Biology, 2017, 93(4):341-353.
[41] Kosmiatin M, Martasari C, Yunimar, et al. In vitro selec-tion to increase huanglongbing tolerance of Citrus de-rived from in vitro breeding[J]. IOP Conference Series:Earth and Environmental Science, 2020, 457(1):012080.
[42] Deng H, Achor D, Exteberria E, et al. Phloem regenera-tion is a mechanism for huanglongbing-tolerance of "Bearss" lemon and "LB8-9" Sugar Belle® mandarin[J]. Frontiers in Plant Science, 2019, 10:277.
[43] Cheng C Z, Zhong Y, Wang B, et al. The upregulated ex-pression of the Citrus RIN4 gene in HLB diseased Cit-rus aids Candidatus Liberibacter asiaticus infection[J]. In-ternational Journal of Molecular Sciences, 2022, 23(13):6971.
[44] 武晓晓,娄兵海,唐艳,等.耐(抗)柑橘黄龙病种质材料的评价研究[J].中国果树, 2021(11):44-49.
[45] 陈仕钦,卢小林,陈玉龙,等.柑橘黄龙病防控药剂筛选试验初报[J].植物保护, 2014, 40(2):166-170.
[46] Archer L, Qureshi J, Albrecht U. Efficacy of trunk inject-ed imidacloprid and oxytetracycline in managing huan-glongbing and Asian Citrus psyllid in infected sweet or-ange (Citrus sinensis) trees[J]. Agriculture, 2022, 12(10):1592.
[47] 韩鹤友,程帅华,宋智勇,等.柑橘黄龙病药物防治策略[J].华中农业大学学报, 2021, 40(1):49-57.
[48] 黄洋,关巍,王铁霖,等.两类抗生素药剂对‘纽荷尔'脐橙黄龙病菌的抑制作用及根际细菌群落结构的影响[J].植物保护, 2021, 47(6):83-92.
[49] 孟华岳,郑淑琼,文英杰,等.滴灌施用噻虫胺防治柑橘木虱研究[J].华南农业大学学报, 2019, 40(2):47-52.
[50] Roldan E L, Stelinski L L, Pelz-Stelinski K S. Foliar an-tibiotic treatment reduces Candidatus Liberibacter asiati-cus acquisition by the Asian Citrus psyllid, Diaphorina citri (Hemiptera:Liviidae), but does not reduce tree in-fection rate[J]. Journal of Economic Entomology, 2023, 116(1):78-89.
[51] Huang C Y, Araujo K, Sánchez J N, et al. A stable anti-microbial peptide with dual functions of treating and pre-venting Citrus huanglongbing[J]. Proceedings of the Na-tional Academy of Sciences of the United States of America, 2021, 118(6):e2019628118.
[52] Widyaningsih S, Joko T, Utami S N H, et al. The effec-tiveness and bacterial communities of biofertilizer appli-cation for huanglongbing disease control in indonesia[C]//International Symposia on Horticulture. Bali, Indonesia:Filodiritto Editore, 2018:80-88.
[53] Munir S, Li Y M, He P B, et al. Defeating huanglong-bing pathogen Candidatus Liberibacter asiaticus with in-digenous Citrus endophyte Bacillus subtilis L1-21[J]. Frontiers in Plant Science, 2022, 12:789065.
[54] Maluta N, Castro T, Lopes J R S. Entomopathogenic fun-gus disrupts the phloem-probing behavior of Diaphorina citri and may be an important biological control tool in Citrus[J]. Scientific Reports, 2022, 12:7959.
[55] 周雅婷.柑橘木虱寄生天敌亮腹釉小蜂的生物学基础研究[D].广州:华南农业大学, 2016.
[56] Gabriel D, Gottwald T R, Lopes S A, et al. Bacterial pathogens of Citrus:Citrus canker, Citrus variegated chlorosis and huanglongbing[M]//The Genus Citrus. Am-sterdam:Elsevier, 2020:371-389.
[57] Zhang Y, Chen Y, Ma J, et al. Controlling Citrus huan-glongbing based on soil remediation and biocontrol[J]. European Journal of Plant Pathology, 2024, doi:10.1007/s10658-024-02835-y.
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