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2025 , Vol. 43 >Issue 11: 97 - 106

DOI: https://doi.org/10.3981/j.issn.1000-7857.2023.11.01686

研究论文

多组学研究探索抑制胃癌新疗法

  • 谢丽 , 1 ,
  • 施文艳 1 ,
  • 李鑫琳 1 ,
  • 熊娟娟 1 ,
  • 刘晶晶 1 ,
  • 姚宏亮 1 ,
  • 周程远 1 ,
  • 黄维 , 2, *
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  • 1. 金陵科技学院动物科学与食品工程学院, 南京 210038
  • 2. 南京中医药大学附属医院/江苏省中医院, 南京 222000
黄维（通信作者），主治医师，研究方向为肿瘤放疗及临床，电子信箱：

谢丽，讲师，研究方向为多组学检测及肿瘤模型，电子信箱：

收稿日期: 2023-11-16

  网络出版日期: 2025-06-26

基金资助

2025年江苏省高等学校大学生创新创业训练计划项目(202513573098Y)

金陵科技学院“创客”虚拟班项目(2023006)

金陵科技学院高层次引进人才科研启动项目(jit−b−202157)

江苏省中医院科技项目(Y22009)

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Multiomics research explores new therapies for inhibiting gastric cancer

  • Li XIE , 1 ,
  • Wenyan SHI 1 ,
  • Xinlin LI 1 ,
  • Juanjuan XIONG 1 ,
  • Jingjing LIU 1 ,
  • Hongliang YAO 1 ,
  • Chengyuan ZHOU 1 ,
  • Wei HUANG , 2, *
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  • 1. College of Animal Science and Food Engineering, Jinling Institute of Technology, Nanjing 210038, China
  • 2. Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 222000, China

Received date: 2023-11-16

  Online published: 2025-06-26

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All rights reserved. Unauthorized reproduction is prohibited.
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摘要

选取人胃癌BGC-823细胞系为模型，通过多组学研究方法，探讨丁酸盐和B族维生素联用对癌细胞生长的影响及可能的作用机制。通过cck-8方法观察维生素B2与丁酸盐联合作用对胃癌细胞BGC-823的抑制作用，Chou-Talay中效分析法评价两组分的联合效应，并以光学显微镜观察细胞形态变化；通过流式细胞术观察维生素B2和丁酸盐联用对BGC-823细胞凋亡及细胞周期的影响；以Western blot方法测定抗癌基因蛋白PTEN及其下游PI3K蛋白表达情况；通过对荷瘤裸鼠模型的作用，观察二者联合体内抑瘤效果；通过胃癌组和健康人群对照组血清中多代谢物的代谢组学分析建立多元判别模型，初步筛选差异代谢物，并在这些代谢物基础上进行通路聚集分析和代谢途径分析。结果表明，cck-8细胞活力和Chou-Talay中效分析结果证明维生素B2可协同增强丁酸盐对BGC-823细胞的杀伤作用，且作用过程中影响了细胞分化，导致细胞形态变大，分裂受阻；流式细胞术显示维生素B2与丁酸盐联用可显著增加胃癌细胞的凋亡比例，将细胞周期阻滞在G2/M期；Western blot结果显示维生素B2和丁酸盐联用可使PTEN蛋白水平显著上升，其下游PI3K表达水平则显著下降；体内荷瘤裸鼠模型显示维生素B2和丁酸盐联用可显著抑制肿瘤的生长；人群代谢组学分析建立多元OPLS-DA模型，并提示丁酸和维生素B2均为胃癌患者体内显著差异代谢物；通路富集分析和途径分析显示脂肪酸和B族维生素的代谢通路在胃癌患者体内具有重要的作用。维生素B2和丁酸盐联用可协同增强对胃癌的抑制作用。

关键词： 丁酸盐; 维生素B2; 胃癌细胞; 协同作用

本文引用格式

谢丽 , 施文艳 , 李鑫琳 , 熊娟娟 , 刘晶晶 , 姚宏亮 , 周程远 , 黄维 . 多组学研究探索抑制胃癌新疗法[J]. 科技导报, 2025 , 43(11) : 97 -106 . DOI: 10.3981/j.issn.1000-7857.2023.11.01686

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