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2025 , Vol. 43 >Issue 12: 138 - 152

DOI: https://doi.org/10.3981/j.issn.1000-7857.2025.04.00062

特色专题

环状适配体的合成、功能与应用

  • 姚丽丽 ,
  • 刘涛 ,
  • 毛瑜 , * ,
  • 郑磊 , *
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  • 合肥工业大学食品与生物工程学院, 合肥 230009
毛瑜（共同通信作者），副研究员，研究方向为功能核酸的体外筛选及其在生物医学与食品安全检测中的应用，电子信箱：
郑磊（通信作者），教授，研究方向为食品营养与安全以及物质科学与生命科学，电子信箱：

姚丽丽，博士研究生，研究方向为环状适配体的体外筛选及其在食品安全中的应用，电子信箱：

收稿日期: 2025-04-14

  网络出版日期: 2025-07-03

基金资助

国家自然科学基金区域创新发展联合基金重点项目(U23A20265)

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Synthesis, functions and applications of circular aptamers

  • Lili YAO ,
  • Tao LIU ,
  • Yu MAO , * ,
  • Lei ZHENG , *
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  • School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China

Received date: 2025-04-14

  Online published: 2025-07-03

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All rights reserved. Unauthorized reproduction is prohibited.
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摘要

环状适配体作为一类具有闭合环状拓扑结构的核酸分子，凭借其独特的核酸外切酶抗性、优异的热力学稳定性及与滚环扩增技术的高度兼容性，已成为食品安全、环境监测及疾病诊断领域的优良分子识别探针。系统阐释了环状适配体的合成方法、高效筛选策略、结构与功能理性设计、滚环扩增驱动超灵敏检测与多价探针应用，并对环状适配体的大规模高效合成技术、人工智能辅助结构设计以及面向复杂生物基质互作解析的动态筛选模型开发的未来发展趋势进行了展望。

关键词： 环状适配体; 筛选; 结构设计; 合成方法; 滚环扩增

本文引用格式

姚丽丽 , 刘涛 , 毛瑜 , 郑磊 . 环状适配体的合成、功能与应用[J]. 科技导报, 2025 , 43(12) : 138 -152 . DOI: 10.3981/j.issn.1000-7857.2025.04.00062

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