通过研究围绝经期综合征舌苔脱落细胞调控蛋白与肝郁病理的相关性，为临床诊疗提供实验依据。本研究采用随机数字表法，选取50例健康妇女作为对照组，150例围绝经期综合征患者作为观察组，证素辨证计算肝郁积分，巴氏染色检测舌苔脱落细胞的成熟指数（MI）和成熟价值（MV），流式细胞仪检测舌苔脱落细胞凋亡指数，免疫组化检测舌苔脱落细胞调控蛋白。结果显示，围绝经期综合征肝郁积分明显高于健康妇女；肝郁病理改变，与舌苔脱落细胞MI的中层细胞数量呈正相关，与舌苔脱落细胞MI的表层细胞数量及MV呈负相关；肝郁病理改变，与舌苔脱落细胞凋亡指数、Fas、Bax的阳性率呈正相关，与舌苔脱落细胞中Bcl-2的阳性率呈负相关。提示肝郁病理是围绝经期综合征的核心病机之一，肝郁病理与舌苔脱落细胞的成熟度、凋亡指数及凋亡调控蛋白相关。
The interrelationship between tongue epithelial cell apoptosis regulation of portein and pathological changes of liver depression in perimenopausal syndrome is investigated to provide a solid experimental basis for related clinical diagnosis and treatment. 50 cases of healthy women and 150 cases of perimenopausal syndrome patients were randomly divided into the control group and experimental groups. Liver depression points were evaluated by syndrome element differentiation; Maturation Index (MI) and Maturation Value (MV) of tongue epithelial cells were determined by Papanicolaou staining; the apoptotic rate of tongue epithelial cells was measured by flow cytometric; and the apoptosis regulation of portein was determined by immunohistochemical. The results show that liver depression points of syndrome patients are significantly higher than those of healthy women; pathological changes of liver depression, and the middle cells MI of tongue epithelial cells are positively correlated, and the surface cells MI and MV of tongue epithelial cells are negatively correlated; pathological changes of liver depression, and apoptotic rate, Fas, Bax of tongue epithelial cells are positively correlated, and Bcl-2 of tongue epithelial cells are negatively correlated. It is concluded that the liver depression is one of the cores of pathogenesisa in the perimenopausal syndrome, and the tongue epithelial cells maturity, apoptotic rate and apoptosis related regulation of gene are interrelated.
