采用不同浓度NaCl溶液处理盐生药用植物黑果枸杞幼苗,通过测定细胞质膜透性(相对电导率)、丙二醛(MDA)、脯氨酸(Pro)和可溶性糖等的变化,探讨黑果枸杞幼苗的耐盐机制。结果表明,叶片中的相对电导率和丙二醛含量随NaCl浓度的提高和胁迫时间的延长逐渐增大,且高浓度盐处理下质膜伤害程度和MDA积累幅度均相对较大;脯氨酸含量随NaCl浓度的增加而大幅增加,其中,在胁迫第6天,200、300、400和500mol/L NaCl处理的增加幅度分别高达405.78%、800.11%、773.78%和747.51%,同时,随着胁迫时间的延长表现出先增加后下降的倒“V”型趋势,但总体还是呈现增加态势;可溶性糖含量在胁迫初期,随着盐浓度的增加呈现先下降后上升的“V”型态势,且随胁迫时间的增加,各处理叶片中可溶性糖含量的变化规律与脯氨酸的一致,但其最大值出现在胁迫第18天,比胁迫初期分别增加64.62%、83.15%、106.60%、207.00%和186.70%;比较而言,在盐胁迫下,黑果枸杞幼苗叶片中脯氨酸含量积累较为敏感,是其适应逆境环境的一种有效方式。本研究认为,在盐胁迫下,黑果枸杞可以通过在其体内积累大量的有机渗透物质以适应外界不利环境。
The changes of several physiological indexes including the relative permeability of membrane, MDA, proline and soluble sugar are studied at the seedling stage of medicinal halophyte Lycium ruthenicum under different NaCl stress levels in order to understand better the mechanism of its salt tolerance. It is shown that the relative conductivity and the MDA content increase with the increase of NaCl concentration and stress time, and the increase rate is relatively higher under higher NaCl concentration than lower concentration. The content of proline also increases significantly with the increase of NaCl concentration, and the increases under 200, 300, 400 and 500mol/L NaCl stress are 405.78%, 800.11%, 773.78% and 747.51%, respectively. In addition, the content of proline first increases and then decreases with the stress time. At the initial stage of salt stress, the content of soluble sugar first decreases and then increases with the increase of salt concentration. With the increase of stress time, the variation trend of soluble sugar is consistent with that of proline, but the maximum value appears at the 18th day after salt stress, and the increases under different NaCl concentrations are 64.62%, 83.15%, 106.60%, 207.00% and 186.70%, respectively, as compared with that at the initial stage. Relatively speaking, the accumulation of proline in leaves of Lycium ruthenicum is more sensitive to salt stress, which is an effective mode to adapt the environmental stress. In conclusion, Lycium ruthenicum could absorb a great amount of soluble organic osmotica to adapt harmful environment.