采用粉末注射成形(MIM)技术制备孔隙度分别为30%、40%、50%、60%的多孔钛种植体,并通过酸碱热处理及仿生沉积羟基磷灰石(HA)进行表面改性。将4组材料与体外培养的成骨细胞MG63联合培养,测定不同孔隙度的多孔钛种植体对MG63增殖、黏附、分化的影响。将4组材料分别植入狗股骨内12周后,制作硬组织切片,用Goldner's三色法染色,测定不同孔隙度的多孔钛种植体对骨整合的影响。结果显示60%的组多孔钛种植体在成骨细胞增殖数量、黏附形态、碱性磷酸酶(ALP)及骨钙素(OCN)含量、骨性结合的形成等方面均优于其他组。研究表明,改性后具有连通孔结构的高孔隙度的多孔钛种植体有利于成骨细胞的增殖、黏附、分化,且动物试验显示该种植体有利于骨整合,从而为制备新型多孔钛种植体提供理论依据,为种植义齿的发展提供新的理论基础。

This paper discusses the preparation of new porous titanium implants with different porosities and the influence of osteoblast cells on adhesion, proliferation, differentiation and osseointegration. The metal injection molding (MIM) is used to prepare the porous titanium implants with porosities of 30%, 40%, 50%, 60%, followed by the surface modification by means of acid-base heat treatment, and biomimetic deposition hydroxyapatite. With four groups of materials cultured with MG63, the influences of different porosities of the porous titanium implant on the adhesion, the proliferation and the differentiation of osteoblast cells MG63 in vitro are studied. With the four groups of materials implanted to the femoral of dogs for 12 weeks, the hard tissue slice and dye are made by using the Goldner's three color method, to study the effect of osseointegration. The results show that the 60% of cases of a group of materials are better than the other three groups on four aspects: the osteoblast quantity, the osteoblast stick form, the content of ALP and OCN and the formation of the bony combination. It is indicated that the high porosity implants after the surface modification with connected holes are propitious to the osteoblast cell's adhesion, the proliferation and the differentiation. They are also propitious to the osseointegration. This study provides a theoretical basis for the development of new porous titanium implants and implant dentures.