Determination of Musk Ketone Content in Cosmetics by Reversed-phase High Performance Liquid Chromatography
CHEN Zhirong1, GAO Xiaoxuan2, MU Min3, ZHANG Pengxiang2, ZHAO Hua2
1. National Institutes for Food and Drug Control, Beijing 100050, China;2. School of Science, Beijing Technology and Business University, Beijing 100048, China;3. China Association of Fragrance Flavor and Cosmetic Industries, Beijing 100079, China
Abstract：Due to the human carcinogenic effect of musk ketone it is the restriction ingredient in cosmetics, and the content analysis of musk ketone is necessary. The various matrixes of cosmetics are prepared by using ultrasonic-assisted extraction. A reversed-phase high performance liquid chromatography method for the determination of musk ketone in cosmetics is established. The samples are analyzed under the condition of Kromasil-C18 column (250mm×4.6mm i.d., 5μm) with UV 235nm. The mobile phase is a gradient elution with V(CH3CN):V(H2O) = 80:20 at a flow rate of 1.0mL/min. Results show that as the concentration of musk ketone is in the range of 0.5-150μg/mL, the relationship between concentration and the corresponding peak area presents the good linearity (r=0.9998). The regression equations could be expressed as A=13500ρ-4829; and the recovery ratio of musk ketone achieves at 90.5%-102.5%. The RSD of the method is 0.45%-0.98%. Therefore, an accurate and stabilizing method with extensive applications is proposed and built. The method provides the reference for the analysis on musk ketone and for the establishment of the state regulations.
2011, Vol. 29 
