石油类污染物进入土壤环境，会对土壤的理化性质、微生物活性等产生危害，进而影响土壤呼吸强度。以某石油类污染场地为研究对象，通过调查取样，采用分子生物学手段对污染发生后包气带、含水层的土壤现状微生物量、土壤化学指标、基础呼吸作用进行研究。研究发现，土壤呼吸强度首要受微生物量影响，其次受硝酸盐、氨氮、亚硝酸盐等因子影响。石油污染对土壤基础呼吸强度的影响，主要体现在对浅层土壤（0.5 m）、地下水位变动带（1.5 m）的土壤基础呼吸强度具有一定的抑制作用。
The petroleum pollutants permeating into the soil environment will affect the soil physical and chemical properties and microbial activity, which in turn affects soil respiration intensity. Some certain pollution area is taken as the research object in this paper, and through surveying and sampling，and by means of molecular biology method, the soil microbial biomass, soil chemical indicators, and soil basal respiration of the polluted vadose and aquifer are studied. It is shown that the basal soil respiration is mainly affected by microorganism quantity, followed by nitrate, ammonia nitrogen, nitrite, etc. The influence of oil pollution on soil basic respiration is mainly the inhibitory effect in the shallow soil depth (0.5 m) and in the underground water level change area (1.5 m).
[1] 魏媛, 张金池, 俞元春, 等. 退化喀斯特植被恢复过程中土壤基础呼吸 及代谢熵的变化[J]. 土壤通报, 2010, 41(4): 787-801. Wei Yuan, Zhang Jinchi, Yu Yuanchun, et al. Changes of soil basal respiration and qCO2 along successional processes of degraded karst vegetation[J]. Chinese Journal of Soil Science, 2010, 41(4): 787-801.
[2] 施政, 汪家社, 何容, 等. 武夷山不同海拔土壤呼吸及其主要调控因子[J]. 生态学杂志, 2008, 27(4): 563-568. Shi Zheng, Wang Jiashe, He Rong, et al. Soil respiration and its regulating factor along an elevation gradient in Wuyi Mountain of Southeast China[J]. Chinese Journal of Ecology, 2008, 27(4): 563-568.
[3] Kirschbaum M U F. The temperature dependence of soil organic matter decomposition and the effect of global warming on soil organic C storage[J]. Soil Biology & Biochemistry, 1995, 27(6): 753-760.
[4] Schlesinger W H, Andrews J A. Soil respiration and the global carbon cycle[J]. Biogeochemistry, 2000, 48(1): 7-20.
[5] 刘颖, 韩士杰. 长白山四种森林土壤呼吸的影响因素[J]. 生态环境学, 2009, 18(3): 1061-1065. Liu Ying, Han Shijie. Factors controlling soil respiration in four types of forest of Changbai Mountains, China[J]. Ecology and Environmental Sciences, 2009, 18(3): 1061-1065.
[6] 王威, 苏小四, 张玉玲, 等. 石油类污染场地的自然衰减作用[J]. 吉林 大学学报: 地球科学版, 2011, 41(S1): 310-314. Wang Wei, Su Xiaosi, Zhang Yuling, et al. Natural attenuation of a petroleum contaminated site[J]. Journal of Jilin University: Earth Science Edition, 2011, 41(S1): 310-314.
[7] 姚槐应, 黄昌勇. 土壤微生物生态学及其实验技术[M]. 北京: 科学出 版社, 2006. Yao Huaiying, Huang Changyong. Microbial ecology and microbial ecology experimental techniques[M]. Beijing: Science Press, 2006.
[8] 张海燕, 张旭东, 李军, 等. 土壤微生物量测定方法概述[J]. 微生物学 杂志, 2005, 25(4): 95-99. Zhang Haiyan, Zhang Xudong, Li Jun, et al. Outline of soil microbial biomass measurement methods[J]. Journal of Microbiology, 2005, 25 (4): 95-99.
[9] 陶水龙, 林启美, 赵小蓉. 土壤微生物量研究方法进展［J］. 土壤肥料, 1993, 12(6): 15-18. Tao Shuilong, Lin Qimei, Zhao Xiaorong. Advances in microbial biomass research methods[J]. Soil and Fertilizer, 1993, 12(6): 15-18.
[10] 元炳成, 刘权, 黄伟, 等. 镁碱化盐土微生物生物量和土壤基础呼吸[J]. 土壤, 2011, 43(1): 67-71. Yuan Bingcheng, Liu Quan, Huang Wei, et al. Microbial biomass and basal soil respiration in alkalized magnesic soils[J]. Soils, 2011, 43(1): 67-71.
[11] 苏慧敏, 李叙勇, 欧阳扬. 土壤微生物量和土壤呼吸对降雨的响应[J]. 生态环境学报, 2011, 20(10): 1399-1402. Su Huimin, Li Xuyong, Ouyang Yang. Responses of soil microbial biomass and soil respiration to rainfall[J]. Ecology and Environmental Sciences, 2011, 20(10): 1399-1402.
