通过与水浴回流法比较，建立了全自动加压溶剂萃取法，可简单、快速测定洋葱中保健活性物质芦丁和槲皮素含量。采用高效液相色谱仪在368nm波长处测定芦丁和槲皮素含量。色谱柱为C18柱，流动相为甲醇-磷酸溶液（58:42，体积比），以1.0ml/min流速等度洗脱，外标法定量。结果表明，在芦丁和槲皮素质量浓度为0.1~200mg/L时，其线性相关系数均大于0.999。在2.0~100mg/kg的添加水平下，芦丁和槲皮素的平均回收率分别为83.5%~94.2%和84.8%~91.2%；相对标准偏差分别为2.9%~7.4%和4.2%~6.9%（n=6）。全自动加压溶剂萃取法的检出限（LOD），芦丁和槲皮素分别为0.05，0.03mg/kg。定量限（LOQ），芦丁和槲皮素分别为0.2，0.1mg/kg。研究建立的运用全自动加压溶剂萃取仪提取、高效液相色谱测定洋葱中芦丁和槲皮素的方法，线性良好，重现性较好，灵敏度较高，能够满足蔬菜和水果等农产品中芦丁和槲皮素的快速检测。
As compared with the water circumfluence method, a simple and rapid method was developed for the determination of contents of rutin and quercetin in onion by Automated Pressurized Liquid Extractor (APLE). The contents of rutin and quercetin were first determined by High Performance Liquid Chromatography (HPLC) in 368nm and analyzed on the C18 column by a isocratic elution, using a mixture of methanol-phosphoric acid solution(58:42, V/V) as the mobile phase with a flow rate of 1.0mL/min. The method is calibrated by an external standard method. Under the optimal conditions, the correlation coefficient of rutin and quercetin in the concentration range of 0.1~200mg/L is more than 0.999. The average recovery rates of rutin and quercetin in the spiked range of 2.0~100mg/kg are 83.5%~94.2% and 84.8%~91.2%, respectively. And the relative standard deviations are 2.9%~7.4% and 4.2%~6.9%, respectively (n=6). Using the automated pressurized liquid extractor method, the detection limit (LOD) of rutin and quercetin are 0.05mg/kg and 0.03mg/kg, respectively, and the quantitative limits (LOQ) of rutin and quercetin are 0.2mg/kg and 0.1mg/kg, respectively. Therefore, the method developed for the determination of rutin and quercetin in onion by automated pressurized liquid extractor and high performance liquid chromatography gives a good linearity, repeatability and high sensitivity, and is suitable for the rapid determination of rutin and quercetin in vegetables and fruits.
