To establish the model by cigarette smoking and stimulus of cold and dryness environment, the Enzyme-linked immunoadsordent assay method was used to measure MMP-9 and TNF-α content in rat's blood serum and HA, LN in BALF, fluorescence quantitative PCR to determine the expression of MMP-1mRNA in bone. The results were analyzed by using SPSS 11.5 statistical software to see the effects of cigarette smoking and cold dryness in Xinjiang' environment on matrix metalloproteinase -9 (MMP-9) and tumor necrosis factor-α (TNF-α) in rat's serum, HA, LN in BALF , and MMP-1 mRNA in rat's bone. The results show that MMP-9 level is significantly higher than that in the control group (P<0.01), between the two groups, TNF-α in serum, HA, LN in BALF, are not significantly different, but the model group's figures are larger. The expression of MMP-1 mRNA is higher in the cold and dry group than in the control group (P<0.05). So it may be concluded that cigarette smoking and cold dryness environment can stimulate the increase of MMP-9 and TNF-α in rat's serum, HA, LN in BALF and MMP-1 mRNA in rat's bone. And this may be part of the mechanism that cigarette smoking and cold dryness environment lead to disorders of the body.