Abstract: Glaucoma is the degeneration of the optic nerve head caused by elevated Intraocular Pressure (IOP) and diurnal variations. It is the second leading cause of blindness. The pathogen of glaucoma is very complex. Although abnormal elevation of IOP is the main reason, visual field defects and optic disc changes in eyes are also the symptoms. Therefore, IOP and fundus are two important and indispensable parameters for the diagnosis of glaucoma. Based on the principle of Goldmann applanation tonometer, the IOP measurement and fundus image are obtained by a single measurement. FPGA is the core unit of this design, the value of IOP and the acquisition of dynamic fundus images are measured by the optical probe which is pushed to contact the eye. IOP is measured by the software of Nios II which controls the probe pressure by using embedded soft-core. The value is indirectly calculated through selected appropriate algorithm of image segmentation for corneal flattening area. Design of dynamic fundus image acquisition system includes lighting system and observation system. The lighting system is made up of Cora lighting. The observation system acquires flattening corneal images and the fundus images in the process by means of imaging sensor. In order to verify the function of this system, the experiments, such as simulated eyeball and live animals, are carried out. The system is able to calculate IOP, acquire real-time dynamic images, and display and store images quickly and easily. It provides two important and indispensable parameters for the diagnosis of glaucoma.