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| Rapid DNA Extraction from Half－grain Rice Dry Seeds for SSR Analysis |
| WANG Hui1, GUO Feng1, GUAN Chao1, DUAN Yuxi2, BAI Hongzhi3 |
| 1. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China;2. Plant Protection College, Shenyang Agricultural University, Shenyang 110866, China;3. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China |
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Abstract: To explore an easy, rapid and economical approach for extracting rice (Oryza sativa L.) genomic DNA for SSR (Simple Sequence Repeat) analysis, half-grain dry seed of rice Shennong265 is taken as tested material, just one step was used for cell lysis by SDS buffer, and the operation of RNA digestion was integrated into protein extraction. During this process, the SDS concn was 0.5% (W/V), water bath time was 10min, extraction with chloroform/isoamyl alcohol was used. High quality genomic DNA from rice seed was obtained simply and quickly. In this paper, the results of agarose gel electrophoresis all showed that the purity and integrity of the isolated DNA were satisfying; the amplified bands were clear as well as good in repeatability and stability, indicating that it can be used in SSR marker analysis. This is a time-saving method for extraction of genomic DNA, and can be effectively applied in genetic diversity analysis of rice seed and marker aided breeding activity.
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Received: 09 April 2013
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2013, Vol. 31 
