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TO INVESTIGATE THE EFFECT OF CRYPTOTANSHINONE ON INSULIN RESISTANT OVARIAL GRANULAR CELLS BY DNA MICROARRY |
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Abstract: The mechanism of Cryptotanshinone in treating ovary insulin resistance is identified in this paper. The ovarian granulose cells from porcine follicles were isolated and cultured in vitro, and insulin resistance was induced by treatment with Wortmannin. The cells were divided into 4 groups, the blank control group cultured with DMSO, the Wortmannin group cultured with Wortmannin 250 nmol/L, the Cryptotanshinone group cultured with Wotrmannin 250 nmol/L as well as 0.02 g/L Cryptotanshinone. After being incubated for 48 h, the total RNA was isolated from cells, and the differently expressed genes were screened from them by the gene chip technique. Then they were analyzed by molecule annotation system to get differential gene expression profiles and pathways of the four groups. The results show that 42 differential genes are screened through the channel gene chip technique, including 22 down-regulation genes and 20 up-regulation genes. These differential expression genes have various biological functions, such as lipid metabolism, intercellular signal transmission and so on. And the Cryptotanshinone may improve ovary insulin resistance by adjusting many genes and signal pathway. It is implied that Cryptotanshinone may be used to treat endocrine disease and reproductive barrier of polycystic ovary syndrome.
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Received: 14 May 2009
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