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| Breeding and Selection of Mixed Bacterial Cultures for Volatile Fatty Acid Production by Rice Straw Fermentation |
| LI Jianzheng, SONG Junling, AI Binling |
| State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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Abstract: To develop genetic resources for microorganisms and to study the volatile fatty acid production by rice straw fermentation, mixed bacterial cultures FMc, FMd, FMs, FMw and FM(d+w) were obtained by subculture with cow muck, pig compost soil, rotten wood, and mixture of pig compost soil and rotten wood as inoculums. Among the five mixed cultures, FMw shows the highest straw degradation rate of 46.4%. The highest specific production rates of the total volatile fatty acid and the total butyric acid from rice straw are reached by the mixed culture FM(d+w) with a value of 0.64 and 0.48g/g, respectively. The mixed culture FMd produces more acetic acid from rice straw than others with a specific yield of 0.35g/g. It is found that the five mixed bacterial cultures all lack the acidity glucanase, resulting in a great restriction in the degradation of rice straw and the fatty acid yield. In order to improve the mixed cultures with respect to their fatty acid yield, a domestication to fatty acids and an optimization of cultivation conditions are desirable.
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Received: 12 June 2012
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2012, Vol. 30 
