Atrazine has become a research hot spot for many years due to its universality, high pollution, and difficult degradation. In the recent years, much research has been done on Atrazine. All sorts of degradation methods have been found. Among them, the bio-degradation is the most effective. Based on atrazine-degrading bacterium, immobilization method, and some materials, a new study is conducted. A kind of biologically active material, namely, perlite-phosphorylated polyvinyl alcohol (perlite-PPVA) is prepared with modified polyvinyl alcohol (PVA) by using phosphate, perlite, and atrazine-degrading bacterium: Pseudomonas W4. The effect of the material on the degradation of atrazine is discussed under the optimal preparation conditions and the other different reactive conditions.The results show that the best preparation conditions are perlite (particle sizeis 0.60-2.00mm)/10% PVA (V/V) =35/65, Pseudomonas W4 embedded volume is 1g/L, phosphate density is 1.25mol/L of perlite-PPVA. External carbon source and phosphorus source are able to promote its degradation ability of atrazine. The effect of perlite-PPVA on the atrazine degradation is better when the pH is in the range of 5.99-9.03, its ability of anti-acid is also better than the free bacterium. It shows that perlite-PPVA is feasible for the removal of atrazine, and could be used as a new material applied to the degradation of atrazine in waste water or soil after further research.