The nucleic acid detection workstation is a high-precision biochemical analytical instrument composed of optical, mechanical, electrical and other components. There are four functional modules in the workstation, including the high throughput magnetic separation system, the micro-volume pipetting system, the accurate temperature control system and the fluorescent signal detection system. According to the requirements of the above systems, a brief introduction is given for the framework of the hardware system. Based on the hardware functions, the software system completes the design of the experimental process using the tree control: an easy to use and good looking experimental property setting interface and a workbench layout with the MFC drawing. Serial communications are carried out through the slave PC's RSC-485 port with multi-threaded monitoring technologies: saving the experimental processes and results, exporting experimental data with the access database programming for their printing.