Novel high intensity TiO₂ nanotube array film was fabricated via electrochemical anodization, and then open-ended TiO₂ nanotubes array membrane was obtained by HF gas etching the bottom of nanotubes. HK-2 cells and HUVEC cells were cultivated on the open-ended TiO₂ nanotube array surface and polymer dialysis film surface, and biomembrane materials which have physiological function are successfully achieved. Cell viability of TiO₂ nanotube array, Polyether Sulfone (PES), mixed cellulose ester, and regenerated cellulose was investigated by using MTT method. Cell morphology on these membranes was examined by using fluorescence microscope and Filed Emission Scanning Electron Microscope (FESEM). The results indicate that TiO₂ nanotube array film is of the most benefit to cell adhesion and cell proliferation, and the cell viability is the highest, and the second place belongs to the PES membrane, the worst case is regenerated cellulose that is unsuitable for cell proliferation and cell adhesion. Fluorescence microscope shows that TiO₂ nanotube array membrane could promote the cell adhesion and cell proliferation batter than polymeric membrane does. It demonstrates that TiO₂ nanotube array film has excellent biocompatibility, overcoming the deficiency of traditional dialysis materials, improving the cell adhesive; and the film is the ideal candidate materials for biological dialysis.