Nitrobenzene wastewater is hardly degraded, highly toxic and quickly diffuse. The treatment of nitrobenzene wastewater with high concentration has been well studied, but there is little study about the treatment of low-concentration nitrobenzene wastewater. Ordinary water treatment technology is unable to treat this kind of water. Long-term drinking can be seriously harmful on human health and ecological environment is destroyed. In this article, the synthesis of β-cyclodextrin polymers and the adsorption of β-cyclodextrin polymers on nitrobenzene at low concentration were studied, and the effects of solid-to-liquid ratio with pH were discussed. In addition, regeneration of β-cyclodextrin polymers is also discussed. Results show that in the per 100mL solution containing 100mg cyclodextrin polymers, the effects of solid-to-liquid ratio is the best; The adsorption efficiency of nitrobenzene solution can reach more than 80% in solution of 200μg/L, 500μg/L, 1mg/L, 2mg/L; The best adsorption time is 2h;pH has little effect on the adsorption efficiency; The ethanol is selected as the best regenerative agent, which retains the regeneration of 80% after four times.