The study of space biology becomes significant and requires the advanced technologies for stimulating space environment considering the rare opportunity and the high costs of space experiments. In this article, the characteristics and applications of the existing technologies for stimulating the microgravity environment are systematically analyzed. Based on the research tendency of the coupling effects of multi-factors, laser trap is proposed to be the next generation technology for the simulation. This technology has the great advantages for manipulating cells without contact, manipulating intra-cell particles and fixing cell position, which is valuable for producing the good simulation effect. The well compatibility and complementation between the technologies of laser trap and the single ion radiation will be very useful for the study of coupling effects of the microgravity and radiation.