Though comparing the toxicity of the Gansuibanxia soup, Gansuibanxia soup with Zhigancao removed, Gansuibanxia soup with Cugansui removed, Gansuibanxia soup with Zhigancao and Cugansui removed, and Zhigancao, Cugansui and the compatibility of Zhigancao and Cugansui, the toxicity of single herbs, anti-drug combinations, and the compound containing anti-drug combination is evaluated by dismantling the compound containing anti-drug combination of Gansui and Gancao. The Kunming male mice of 18-20g were randomly divided into a blank group, Gansuibanxia soup group, Gansuibanxia soup with Zhigancao removed group,Gansuibanxia soup with Cugansui removed group, Gansuibanxia soup with Zhigancao and Cugansui removed group, Zhigancao group, Cugansui group and the compatible group of Zhigancao and Cugansui. The blank group was administered with distilled water and other groups were administered with related drugs. For each group, LD₅₀ is obtained in this way: The drugs are continuously administered for 28 days, to cause half of the lethal to evaluate the toxicity. The results show that the values of LD_50(n) for the compatible group of Zhigancao and Cugansui, the Zhigancao group, the euphorbia and pinellia soup group are: 44.21, 48.26, 51.01g crude drug·kg^-1, and for other experimental groups, the half lethal dose is not reached in the case of the continuous administration for 28 days. It is suggested that the Gansuibanxia soup with Gansui and Gancao removed, the anti-drug combination or individually with Gansui or Gancao removed, alone do not show the cumulative toxicity, while for the two flavor combination of anti-drug application in the compound, the LD_50(n) value has an increasing trend.