Articles

Construction of Lentivirus-mediated HPV16-E7 Gene RNAi Cell Model

  • ABUDUKADEER Abida;HASIMU Axiangu;AINIWAER Saimire;ABULIZI Abudula;SHENG Lei
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  • 1. The First Afiliated Hospital, Xinjiang Medical University, Urumqi 830011, China;2. College of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China;3. Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi 830011, China

Received date: 2013-06-28

  Revised date: 2013-11-05

  Online published: 2013-12-18

Abstract

The HPV16-E7 ggene RNAi cell model was constructed. A recombinant lentiviral siRNA expression vector was constructed using a HPV16-E7 oncogene specific siRNA fragment, and an RNAi cell model stably expressing the HPV16-E7-siRNA was established by transfection of 293FT virus-packiging cells with the viral vector followed by infection of HPV16-positive SiHa carcinoma cells with recombinant virus, antibiotica selection and molecular biological characterization. RNAi cell model was successfully established, stably transcribing the HPV16-E7-siRNA fragment, and the inhitory effect on target gene (E7) was also characterized by western blotting and RT-PCR. The RNAi cell model expressing the HPV16-E7 gene-specific siRNA fragment using lentiviral vector was stable, effective and feasible, which lays the foundation for the role study and carcinogenic mechanism of E7 protein.

Cite this article

ABUDUKADEER Abida;HASIMU Axiangu;AINIWAER Saimire;ABULIZI Abudula;SHENG Lei . Construction of Lentivirus-mediated HPV16-E7 Gene RNAi Cell Model[J]. Science & Technology Review, 2013 , 31(35) : 60 -63 . DOI: 10.3981/j.issn.1000-7857.2013.35.011

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