The aims of this study is to set up a high throughput screening model for SHP2 inhibitors and screen the potential inhibitors. By the E. coil expression system GST-SHP2 fusion protein is cloned and over expressed. High-purity SHP2 protein is purified though GSTSepharose column. After establishing the high through screening system with the colorimetric assay of SHP2, a library of 48000 pure compounds is screened using the 384 micro- plate. Among them 75 compounds have inhibitory effects over 50%. Ultimately, three inhibitors are identified as SHP2 inhibitors with high activity. The high throughput screening is a highly sensitive, inexpensive, and operationally simple assay method in identifying SHP2 inhibitors.
GAO Wei
,
SHAO Wei
,
SU Gang
,
LI Jia
,
XIE Xiaodong
. Establishment and Application of High-throughput Screening Model for SHP Inhibitors[J]. Science & Technology Review, 2014
, 32(4-5)
: 91
-94
.
DOI: 10.3981/j.issn.1000-7857.2014.h1.015
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