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Parameter Optimization for Fed Batch Fermentation of Streptomyces lydicus A02 to Produce Natamycin

  • LIU Ting ,
  • LU Caige ,
  • HUA Yupeng ,
  • LIU Dewen ,
  • LIU Weicheng
  • Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received date: 2014-01-27

  Revised date: 2014-03-31

  Online published: 2014-05-13


In order to improve the fermentation process of natamycin by Streptomyces lydicus A02, the main parameters for the fed batch fermentation are optimized. The range of suitable pH values is screened by an assisted optimization using a shaker with the function of automatic feeding to regulate pH values. Then the experiments in 30 L fermentor are carried out by controlling pH values within the suitable range as the main regulation point. The relationship between the natamycin yields and the fermentation parameters is obtained through the software Biostar 5.0. It is shown that the optimized parameter combination for the fed batch fermentation of natamycin by S. lydicus A02 is as follows: The culture temperature is 30℃, the pH value is 6.25-6.29, the DO is 20%-30%, the OUR is 25-15 mmol/(L·h), the CER is 20-12 mmol/(L·h), the amino-N is 0.20-0.23 g/L, and the reducing sugar remains at about 1.5% in 88 h then drops gradually to 0.32% at 112 h, the end of fermentation, when the natamycin yield is up to 2.02 g/L in the supernatant and 6.98 g/L in the total fermented broth. It can be concluded that the fermentation level of natamycin by S. lydicus A02 could be increased effectively by the optimization of the main fermentation parameters.

Cite this article

LIU Ting , LU Caige , HUA Yupeng , LIU Dewen , LIU Weicheng . Parameter Optimization for Fed Batch Fermentation of Streptomyces lydicus A02 to Produce Natamycin[J]. Science & Technology Review, 2014 , 32(12) : 19 -25 . DOI: 10.3981/j.issn.1000-7857.2014.12.002


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