The solid fermentation of Gliocladium roseum ACM941 has not been well studied so far,this paper proposes to improve the fermentation titer of ACM941 through the single factor and orthogonal design. The optimum fermentation conditions are obtained as follows: The bran and cottonseed shell ratio is 4:6, the corn flour 2%, the oat flour 2%, the beef extract 3%, and the peptone 2%. The optimum mycelial growth and sporulation temperatures are 25℃ and 20℃. The initial water content is 30% and the inoculation volume is 6%. Under these conditions, the maximum conidial yield comes to 1.17×109 cfu/g, 12.4 times higher than the initial fermentation level. This provides a support for the development of the biocontrol agent.
ZHANG Taotao
,
LIU Weicheng
,
WU Huiling
,
DONG Dan
,
LIU Ting
. Optimization of Solid Fermentation of Gliocladium roseum ACM941[J]. Science & Technology Review, 2014
, 32(12)
: 26
-30
.
DOI: 10.3981/j.issn.1000-7857.2014.12.003
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