A group of nutrient activators with respect to injection well LU3064 in Xinjiang oil field Hutubi river group are designed, for emulsifying the crude oil. With the bacteria counting method, the MPN method, and the physiochemical property detection method, it is discovered that, in the injection water, upon activation, the concentration of bacteria rises significantly, while its surface tension decreases by 37%. With the molecular biological techniques (16S rDNA clone library), the LU3064 injection water, the LU3036 produced water and the LU3064 activated water are studied. One hundred clones are randomly selected from the clone library to blast in Genbank. The observations show that the injection water is highly diversified, and Alphaproteobacteria is the dominating bacteria. Compared to the injection water, the produced water has a simpler structure, which provides habitats with function bacteria genera such as Pseudomonas, Bacillus, etc. The community structure is found most monotonous in the activation fluid, only Bacillus can be found. Bacillus is discovered in both the injection water and the produced water, but does not dominate in them. The formula successfully activates Bacillus which can be grown in the stratum.
YI Lina
,
CUI Qingfeng
,
MA Yuandong
,
YU Li
,
HUANG Lixin
. Microbial Community and Community Variability after Activated in Luliang Oilfield[J]. Science & Technology Review, 2014
, 32(15)
: 25
-31
.
DOI: 10.3981/j.issn.1000-7857.2014.15.002
[1] 包木太, 孔祥平, 宋永亭, 等. 胜利油田S12块内源微生物群落选择性 激活条件研究[J]. 石油大学学报: 自然科学版, 2004, 28(6): 44-48. Bao Mutai, Kong Xiangping, Song Yongting, et al. Selective activation condition of stratal microflora of S12 block in Shengli Oilfield[J]. Journal of the University of Petroleum: Nature Science Edition, 2004, 28 (6): 44-48.
[2] 罗跃, 佘跃惠. 微生物采油技术研究的国内外动态及本源微生物采油 技术[J]. 国外油田工程, 1999, 8(4): 1-2. Luo Yue, She Yuehui. Dynamics in land and abroad of MEOR and experiments on EOR employing indigenous microorganisms[J]. Foreign Oilfield Engineering, 1999, 8(4): 1-2.
[3] Brown L R. Microbial enhanced oil recovery (MEOR)[J]. Current Opinion in Microbiology, 2010, 13(3): 316-320.
[4] Zheng C, Yu L, Huang L, et al. Investigation of a hydrocarbondegrading strain, Rhodococcus ruber Z25, for the potential of microbial enhanced oil recovery[J]. Journal of Petroleum Science and Engineering, 2012, 81: 49-56.
[5] 李希明, 冯时林, 曾庆坤, 等. 微生物采油技术研究[J]. 油气采收率技 术, 1997(1): 9-18. Li Ximing, Feng Shilin, Zeng Qingkun, et al. Research of microorganism oil recovery technique[J]. Petroleum Geology and Recovery Efficiency, 1997(1): 9-18.
[6] Yamane K, Hattori Y, Ohtagaki H, et al. Microbial diversity with dominance of 16S rRNA gene sequences with high GC contents at 74 and 98 degrees C subsurface crude oil deposits in Japan[J]. FEMS Microbiology Ecology, 2011, 76(2): 220-235.
[7] 包木太, 袁书文, 宋智勇, 等. 不同碳源激活剂对胜利油田中一区油藏 内源菌激活效果评价[J]. 化工学报, 2011(3): 786-791. Bao Mutai, Yuan Shuwen, Song Zhiyong, et al. Evaluation on effects of different carbon source on activating indigenous bacteria in Zhongyi Block of Shengli Oilfield[J]. CIESC Journal, 2011(3): 786-791.
[8] 高配科, 马挺, 赵玲侠, 等. 胜利油田沾3区块内源微生物激活剂的筛 选、优化及效果评价[J]. 化工学报, 2011(7): 2005-2012. Gao Peike, Ma Ting, Zhao Lingxia, et al. Screening, optimization and evaluation of indigenous microorganism activation system in Zhan 3 Block, Shengli Oilfield[J]. CIESC Journal, 2011(7): 2005-2012.
[9] Gittel A, Sorensen K B, Skovhus T L, et al. Prokaryotic community structure and sulfate reducer activity in water from high-temperature oil reservoirs with and without nitrate treatment[J]. Applied and Environmental Microbiology, 2009, 75(22): 7086-7096.
[10] Strapoc D, Picardal F W, Turich C, et al. Methane-producing microbial community in a coal bed of the Illinois basin[J]. Applied and Environmental Microbiology, 2008, 74(8): 2424-2432.
[11] de Weert J P, Vinas M, Grotenhuis T, et al. Degradation of 4-nnonylphenol under nitrate reducing conditions[J]. Biodegradation, 2011, 22(1): 175-187.
[12] 刘秀红, 杨庆, 吴昌永, 等. 不同污水生物脱氮工艺中N2O释放量及 影响因素[J]. 环境科学学报, 2006(12): 1940-1947. Liu Xiuhong, Yang Qing, Wu Changyong, et al. N2O emissions from different biological nitrogen removal processes and factors affecting N2O production[J]. Acta Scientiae Circumstantiae, 2006(12): 1940-1947.
[13] 窦娜莎, 王琳. 16S rDNA克隆文库法分析Biostyr曝气生物滤池处理 城市污水的细菌多样性研究[J]. 环境科学学报, 2011(10): 2117-2124. Dou Nasha, Wang Lin. Bacteria diversity in a biostyr biological aerated filter of municipal wastewater by 16S rDNA[J]. Acta Scientiae Circumstantiae, 2011(10): 2117-2124.
[14] Xia W J, Luo Z B, Dong H P, et al. Synthesis, characterization, and oil recovery application of biosurfactant produced by indigenous Pseudomonas aeruginosa WJ-1 using waste vegetable oils[J]. Applied Biochemistry and Biotechnology, 2012, 166(5): 1148-1166.
[15] 吴小红, 曾光明, 袁兴中, 等. 生物表面活性剂鼠李糖脂对水体中石 油烃降解的促进作用[J]. 应用与环境生物学报, 2006(4): 570-573. Wu Xiaohong, Zeng Guangming, Yuan Xingzhong, et al. Enhancement of Rhamnolipid Biosurfactant for Biodegradation of Petroleum hydrocarbons in water[J]. Chinese Journal of Applied and Environmental Biology, 2006(4): 570-573.
[16] Simpson D R, Natraj N R, McInerney M J, et al. Biosurfactantproducing Bacillus are present in produced brines from Oklahoma oil reservoirs with a wide range of salinities[J]. Applied Microbiology and Biotechnology, 2011, 91(4): 1083-1093.
[17] Sung M H. Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost[J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52(6): 2251-2255.
[18] 夏文杰, 董汉平, 俞理, 等. 嗜热耐盐烃降解菌Geobacillus sp. WJ-2 降解原油性能研究[J]. 中南大学学报: 自然科学版, 2012, 43(1): 8-16. Xia Wenjie, Dong Hanping, Yu Li, et al. Oil-degrading characterization of thermophilic and halotolerant strain Geobacillus sp. WJ-2[J]. Journal of Central South University: Science and Technology Edition, 2012, 43(1): 8-16.
