In this study, Golgi protein 73 (GP73) of hepatocelluler carcinoma (HCC) cell lines with different metastatic potential was purified by antibody affinity chromatography and the purification result was confirmed by LC-MS/MS, providing more proof for GP73 as a biomarker for HCC. Purified GP73 was then analyzed using western blot and lectin blot to obtain its glycosylation level in HCC cell lines with different metastatic potential. We found that glycan of GP73 can be recognized by ConA, AAL, LCA and PHA-E lectins, while the LCA and PHA-E-reactive fraction of GP73 was increased along with the increasing metastatic potential of HCC cell lines. The glycan level of GP73 was different in HCC cell lines with different metastatic potential, and the content of fucose (recognized by LCA) and bisecting structure (recognized by PHA-E) increased in agreement with the metastatic potential.
ZHANG Shu
,
JIANG Kai
,
MAO Xiaoyi
,
LIU Yinkun
. Comparison of GP73 Glycosylation in Human Hepatocellur Carcinoma Cell Lines with Different Metastatic Potential[J]. Science & Technology Review, 2014
, 32(16)
: 15
-19
.
DOI: 10.3981/j.issn.1000-7857.2014.16.001
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