To investigate the association of the cell apoptosis with the expression change of adenine nucleotide translocator (ANT) in the tissue of Uighur women with cervical cancer, we collected cervical tissue samples from Uygur women with cervical lesions (invasive cervical cancer (ICC): 36 cases and cervicitis: 32 cases). The mRNA and protein expression levels of ANT1, ANT2, ANT3, Bax, Bcl-2, and P53 in the tissues from the two groups were examined by real-time fluorescence quantitative PCR (Q-PCR) and western blot (WB). Apoptotic activity of cervical epithelium cells from the two groups was studied by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method. The results of Q-PCR and WB show that compared with the cervicitis group, the expressions of p53 and Bax in the ICC group decreased while the expressions of Bcl-2 and ANT3 increased, and the difference was statistically significant (P<0.05). The TUNEL results showed that the apoptosis index (AI) of the ICC group was obviously higher than that of the normal control cervicitis groups, the difference between the two groups was statistically significant (P<0.05). The abnormal expression of ANT in cervical cancer leads to mitochondrial dysfunction, thus interfering the apoptosis process, which may be an important mechanism of the occurrence and development of cervical cancer.
JIN Hua, MA Xiangyu, GUO Xia, SEYITI Ayinuer, LIU Yan, CHENG Jingxin
. Association of Cell Apoptosis with the Change of Expression of ANT in Tissue of Cervical Cancer[J]. Science & Technology Review, 2014
, 32(16)
: 20
-23
.
DOI: 10.3981/j.issn.1000-7857.2014.16.002
[1] 刘开江, 刘继文, 李晓荣, 等. 新疆维、汉妇女宫颈癌发病危险因素的 流行病学研究[J]. 新疆医科大学学报, 2008, 31(10): 1335-1338. Liu Kaijiang, Liu Jiwen, Li Xiaorong, et al. Epidemiology study of risk factors on Uigur and Han cervical cancer in Xinjiang[J]. Journal of Xinjiang Medical University, 2008, 31(10): 1335-1338.
[2] 拉莱·苏祖克, 彭玉华, 周康, 等. 新疆不同民族宫颈癌发病趋势分析[J]. 新疆医科大学学报, 2006, 29(7): 569-571. Suzuke Lalai, Peng Yuhua, Zhou Kang, et al. The analysis of cervical cancer morbidity of various ethnic groups in Xinjiang[J]. Journal of Xinjiang Medical University, 2006, 29 (7): 569-571.
[3] 张国庆, 刘开江, 赖小军, 等. 新医大附属肿瘤医院1989—2002年住 院病人恶性肿瘤分布[J]. 新疆医科大学学报, 2003, 26(4): 393-395. Zhang Guoqing, Liu Kaijiang, Lai Xiaojun, et al. Distribution of malignant tumor patients in hospital from 1989 to 2002 in the Affiliated Tumor Hospital of Xinjiang Medical University[J]. Journal of Xinjiang Medical University, 2003, 26(4): 393-395.
[4] Jourdain A, Martinou J C. Mitochondrial outer-membrane permeabilization and remodelling in apoptosis[J]. International Journal of Biochemistry & Cell Biology, 2009, 41(10): 1884-1889.
[5] Duarte F V, Gomes A P, Teodoro J S, et al. Dibenzofuran-induced mitochondrial dysfunction: Interaction with ANT carrier[J]. Toxicology in Vitro, 2013, 27(8): 2160-2168.
[6] Cirri P, Chiarugi P. Tumors and their stroma: Mitochondria at the crossroad[J]. Cell Cycle, 2013, 12(2): 204-205.
[7] Lipponen P, Aaltomaa S. Apoptosis in bladder cancer as related to standard prognostic factors and prognosis[J]. Journal of Pathology, 1994, 173(4): 333-339.
[8] Lipponen P, Aaltomaa S, Kosma V A. Apoptosis in breast cancer as related histopathological characteristics and prognosis[J]. European Journal of Cancer, 1994, 30A(14): 2068-2073.
[9] Brambilla E, Negoescu A, Gazzeri S, et al. Apoptosis related factors p53, Bcl-2, and Bax in neuroendocrine lung tumors[J]. American Journal of Pathology, 1996, 149(6): 1941-1952.
[10] Vyssokikh M Y, Brdiczka D. The function of complexes between the outer mitochondrial membrane pore (VDAC) and the adenine nucleotide translocase in regulation of energy metabolism and apoptosis[J]. Acta biochimica Polonica, 2003, 50(2): 389-404.
[11] Liu Y, Chen X J. Adenine nucleotide translocase, mitochondrial stress, and degenerative cell death[J]. Oxid Med Cell Longevity, 2013, 2013: ID146860.
[12] Halestrap A P, Brenner C. The adenine nucleotide translocase: Acentral component of the mitochondrial permeability transition pore and key player in cell death[J]. Current Medicinal Chemistry, 2003, 10(16): 1507-1525.
[13] Gallerne C, Touat Z, Chen Z X, et al. The fourth isoform of the adenine nucleotide translocator inhibits mitochondrial apoptosis in cancer cells[J]. International Journal of Biochemistry & Cell Biology, 2010, 42(5): 623-629.
[14] Belzacq A S, Brenner C. The adenine nucleotide translocator: A new potential chemotherapeutic target[J]. Current Drug Targets, 2003, 4 (7): 517-524.
[15] Sharaf el dein O, Mayola E, Chopineau J, et al. The adenine nucleotide translocase 2, a mitochondrial target for anticancer biotherapy[J]. Current Drug Targets, 2011, 12(6): 894-901.
[16] Belzacq A S, Vieira H L, Verrier F. Bcl-2 and Bax modulate adeninenucleotide translocase activity[J]. Cancer Reseach, 2003, 63: 541-546.
[17] Chiu T L, Su C C. Curcumin inhibits proliferation and migration by increasing the Bax to Bcl-2 ratio and decreasing NF-kappaBp65 expression in breast cancer MDA-MB-231 cells[J]. International Journal of Molecular Medicine, 2009, 23(4): 469-475.
