This paper studies the effect of Helicobacter pylori (Hp) infection on gut microbiota using fresh stool specimens collected from normal persons (Hp negative) and patients with Hp infection. Hp, E. coli O157, Lactobacillus, Enterococcus and Bifidobacterium were amplified by PCR. The expression of Hp was not found in Hp negative group, consistent with the 13C breath test. The expression of Lactobacillus, Enterococcus and Bifidobacterium in Hp negative group was higher than that in Hp infection group. There was no E. coli O157 in Hp negative group. The numbers of Lactobacillus, Enterococcus and Bifidobacterium were changed by Hp infection.
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