Molecular cloning and sequence analysis of sodium-bile acid cotransporter from Echinococcus granulosus in Xinjiang, China

  • YANG Mei ,
  • LIANG Xiaodi ,
  • LI Jun ,
  • LI Liang ,
  • ZHANG Fuchun ,
  • ZHANG Wenbao
  • 1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
    2. Basic Medical College, Xinjiang Medical University, Urumqi 830011, China;
    3. State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China

Received date: 2015-04-13

  Revised date: 2015-06-16

  Online published: 2016-02-04


Sodium-bile acid cotransporter (EgSBACT) functions in the sodium-bile acid transportation in Echinococcus granulosus (Eg) Protoscolex and the sequence is unknown. The specific primers for EgSBACT mRNA are designed and the EgSBACT gene is amplified by RT-PCR from Eg protoscolex total RNA. Then the EgSBACT gene fragment is cloned into pET30a vector which is a prokaryotic expression vector for sequencing and analyzing by bioinformatics method. The PCR products of EgSBACT is about 650 bp whose length is 654 bp and encodes a protein containing 217 amino acids and the pI of this protein is predicted as 9.28. Homology analysis shows that there is 43% of similarity between EgSBACT and the trematode SBACT (GenBank: GAA57409.1). Phylogenetic analysis shows that EgSBACT is clustered with Fluke, and has a lower homology to other species. EgSBACT gene is cloned from protoscolex of Echinococcus granulosus in Xinjiang, which lays a foundation for further study of EgSBACT function in the development and differentiation of PSCs into adult worms. This research provides a way to express the EgSBACT protein in vitro and taking advantage of this protein further research can reveal the exact functions of EgSBACT in the sodium-bile acid transportation in Echinococcus granulosus.

Cite this article

YANG Mei , LIANG Xiaodi , LI Jun , LI Liang , ZHANG Fuchun , ZHANG Wenbao . Molecular cloning and sequence analysis of sodium-bile acid cotransporter from Echinococcus granulosus in Xinjiang, China[J]. Science & Technology Review, 2016 , 34(2) : 215 -220 . DOI: 10.3981/j.issn.1000-7857.2016.2.036


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