To investigate the immunomodulatory effects of sea cucumber oligopeptides and their mechanisms, a total of 250 specific pathogen free (SPF) female BALB/c mice are randomly divided into 5 groups:the control group, the whey protein group (with 0.30 g/kg of whey protein) and the groups with 0.15, 0.30 and 0.60 g/kg of sea cucumber oligopeptides. The experiment takes 30 days. To determine the cell-mediated immunity, the humoral immunity, the macrophage phagocytosis and the natural killer (NK) cell activity in mice, the splenic lymphocyte proliferation and the delayedtype hypersensitivity assays (for the cellmediated immunity), the IgM antibody response of spleen to sheep red blood cells (SRBC) and the serum hemolysin level assays (for the humoral immunity), the carbon clearance assay and the phagocytic capacity of peritoneal cavity phagocytes assays (for the macrophage phagocytosis) and the NK cell activity assays are performed. Furthermore, the flow cytometry is used to determine the T lymphocyte subsets. The results showed that the sea cucumber oligopeptides significantly increase the cellmediated immunity, the humoral immunity, the macrophage phagocytosis and the NK cell activity in mice(P<0.05), and the effects are better than those of the whey protein. From the flow cytometry, it is observed that the sea cucumber oligopeptides significantly enhance the percentages of CD3+ and CD4+ (P<0.05). In conclusion, the sea cucumber oligopeptides could increase the T lymphocyte cell number and the Th cell proportion to enhance the functions of the cellmediated immunity, the humoral immunity, the macrophage phagocytosis and the NK cell activity, and furthermore to improve the immune functions in mice.
HE Lixia
,
CHEN Qihe
,
LIU Rui
,
REN Jinwei
,
SUN Bin
,
LI Lin
,
XU Teng
,
WANG Junbo
,
ZHANG Zhaofeng
,
LI Yong
. Sea cucumber oligopeptides:Immunomodulatory effects and its mechanism[J]. Science & Technology Review, 2016
, 34(11)
: 42
-47
.
DOI: 10.3981/j.issn.1000-7857.2016.11.007
[1] 何丽霞, 李勇.海参寡肽生物学功能研究进展[J].食品科学, 2015(9): 215-218.He Lixia, Li Yong.Biological functions and applications of sea cucum-ber peptide[J].Food Science, 2015(9): 215-218.
[2] Olivera-Castillo L, Perez-Vega J, Gomez-Ruiz J, et al.Release of bio-active peptides by simulated gastrointestinal digestion of sea cucumber protein (Isostichopus Badionotus) [J].Annals of Nutrition and Metabo-lism, 2011, 58: 121.
[3] Bordbar S, Anwar F, Saari N.High-value components and bioactives from sea cucumbers for functional foods-a review[J].Mar Drugs, 2011, 9(10): 1761-1805.
[4] Perez-Vega J A, Olivera-Castillo L, Gomez-Ruiz J A, et al.Release of multifunctional peptides by gastrointestinal digestion of sea cucumber (Isostichopus badionotus) [J].Journal of Functional Foods, 2013, 5(2): 869-877.
[5] 中华人民共和国卫生部.保健食品检验与评价技术规范[S].北京: 中华人们共和国卫生部, 2003.People's Republic of China Ministry of Health.Technical standards for testing & assessment of health food[S].Beijing: People's Republic of China Ministry of Health, 2003.
[6] Yang R, Zhang Z, Pei X, et al.Immunomodulatory effects of marine oli-gopeptide preparation from Chum Salmon (Oncorhynchus keta) in mice [J].Food Chemistry, 2009, 113: 464-470.
[7] 谢永玲, 张明月, 张静, 等.海参寡肽对小鼠的免疫调节作用[J].中国海洋药物, 2009(4): 43-45.Xie Yongling, Zhang Mingyue, Zhang Jing, et al.Immunomodulation of lower polypeptide from sea cucumber in mice[J].Chinese Journal Ma-rine Drugs, 2009, 113: 464-470.
[8] Ahmed A, Saha B, Patwardhan A, et al.The major players in adaptive immunity: 2.Cell-mediated immunity.[J].Resonance, 2009, 14(5): 610-621.
[9] Constant S L, Bottomly K.Induction of Th1 and Th2 CD4+T cell re-sponses: the alternative approaches[J].Annual Review Immunology,1997, 15: 297-322.
[10] Raphael I, Nalawade S, Eagar T N, et al.T cell subsets and their sig-nature cytokines in autoimmune and inflammatory diseases[J].Cyto-kine, 2015, 74(1): 5-17.
[11] Romagnani S.The Th1/Th2 paradigm[J].Immunology Today, 1997, 18(6): 263-266.
[12] Kobayashi M, Fitz L, Ryan M, et al.Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes[J].Journal of Experimental Medicine, 1989, 170(3): 827-845.
[13] Rossol S, Marinos G, Carucci P, et al.Interleukin-12 induction of Th1 cytokines is important for viral clearance in chronic hepatitis B [J].Journal of Clinical Investigation, 1997, 99(12): 3025-3033.
[14] Gately M K, Wolitzky A G, Quinn P M, et al.Regulation of human cy-tolytic lymphocyte responses by interleukin-12[J].Cell Immunology, 1992, 143(1): 127-142.