Organophosphorus pesticides are worldwide widely used to control agricultural and household pests. Overall, organophosphorus compounds account for the 38% of total pesticides used globally. Parathion is a virulent organophosphate insecticide with perfect insecticidal efficiency. However, it is also highly toxic and harmful to human and other creatures. In order to investigate microbial remediation of soil polluted by pesticides, a strain D10 of parathion-degrading is obtained by selective enrichment culture from the pesticides-contaminated soil. Parathion could be used by D10 as a sole carbon source. D10 is identified as Acinetobacter sp. based on the morphological, physiochemical characters and 16S rDNA sequence analyses. The optimal growth temperature for D10 in shaking flasks is 28℃, and the optimal pH is 6.0—7.0. The optimal carbon and nitrogen source are sodium citrate and yeast powder, respectively. D10 grows fast and reaches at the maximum biomass after 24 hours incubation in nutrient medium. The D10-degradation rate of parathion is determined by using the Gas Chromatography(GC), and is 83.1% after 24 hours. The bio-degradation rate of parathion in simulated and sterilized contamination soils with 100 mg·kg^-1 parathion concentration reaches at 66.7% after seven days inoculation. The result shows that the D10 has a great practical value in the remediation of contaminated soils by parathion.