Ovarian cell Insulin Resistance (IR) and abnormal homogenesis is one of the characteristics of Polycystic Ovary Syndrome (PCOS) and could be remedied by the insulin sensitive agent. To investigate herbal medicine as insulin sensitive agents to improve IR ovarian granulosa cell in vitro and clarify the mechanism of herbal medicine on IR, glycyrrhetic acid and silibinin were used to treat dexamethasone (DEX) induced IR porcine ovary granulosa cells. After DEX treatment, the medium testosterone level increased, while the CYP17 mRNA expression increased and the AMP-activated protein kinase activity (AMPK), which is a protein that switches on ATP-producing catabolic pathways and switches off ATP-consuming anabolic processes based on energy status, mRNA expression decreased in vitro IR porcine ovarian theca cell. The results also show that compared to dexamethasone induced IR group, by adding glycyrrhetic acid and silibinin, testosterone production is reduced and mRNA expression of AMPK is increased. In summary, glycyrrhetic acid and silibinin significantly reverse hyperandorgen (testosterone) status and improve the sensitivity of energy balance by promoting the expression of AMPK. Chinese medicine glycyrrhetic acid and silibinin could reduce the hyperandrogenism of in vitro IR granulosa cell via increasing AMPK mRNA expression.