In order to investigate protective effects of polysaccharide from Vitis vinifera L on β-amyloidprotein 25-35(Aβ25-35) induced neurotoxicity in PC12 cells, PC12 cells were treated with Aβ 25-35 in vitro for 24h to establish an Alzheimer's disease (AD) model. Control group, model group (20μmol/L Aβ25-35), and different concentration of VTP groups (20, 40, 80μg/mL) were set up, and cellular morphological change was observed by using the inverted microscope. Cell viability of each group was assayed with Cell Counting Kit-8 (CCK-8) method; the penetration and completion of the cell membrane were detected by means of lactic acid dehydroge-nase (LDH) method; the level of antioxidant enzymes (SOD) and malondialdehyde (MDA) were evaluated by using chromatometry; and apoptotic rates were detected by means of Annexin V-FITC and staining with PI. The results show that comparing with the model group, VTP groups could improve cell injury, the cell numbers of the VTP groups raise obviously, the morphological change of the cell improves significantly, the survival rate of PC12 cells increases; LDH percolation activity decreases, SOD activity increases, MDA level decreases, and the apoptotic rate reduces. In summary, VTP has the protective effects on both oxidative damage and apoptosis of PC12 cells induced by Aβ25-35.