The early-warning plasma biomarkers screening of High-grade cervical Squamous Intraepithelial Lesion (HSIL) for the Uighur women patients by using two-dimensional liquid phase chromatography and mass spectrometry is aimed. The plasma samples from Uighur women with cervical lesions (21 cases with HSIL and 22 cases with cervicitis) were collected and low abundant plasma proteins were prepared. After the establishment of a differential proteome profile by two-dimensional liquid phase chromatography, mass spectrometry and bioinformatics technology were used to identify and analyze differential proteins. Based on the protein isoelectric point gradient and hydrophobic features of low abundant plasma proteins, a differential proteome profile was established for the Uighur women with cervicitis and HSIL; it is found that three peak constituents are upregulated and one is downregulated in the patients with HSIL. Identification of these proteins by mass spectrometry shows that a total of 31 plasma proteins are differentially expressed, four proteins that closely associated with tumor progress including metabolome-related proteins (APOA1), signal transduction-related proteins (mTOR, EphA3), and immune-related proteins (HLA-DQB1) are selected. By focusing on the early-warning low abundant plasma proteome biomarker of Uighur women with precancerous lesion, the evidence for prevention, early diagnosis, and canceration mechanism understanding of cervical cancer in Xinjiang Autonomous Region is provided.