In view of lack of strict attribute and intermediary in drinking water source form and class attribute, nine assessment factors which have much more influence on human health in drinking water source quality assessment are identified. For example, permanganate index, biochemical oxygen demand, total nitrogen, ammonia nitrogen, fluoride, total phosphorus, dissolved oxygen, etc. The ratio of i-th pollutant measured concentration to average value of its each water quality standard is used as the corresponding weight of i-th factor. And all the factors' weights are treated by normalization before taken into the assessment. Then fuzzy comprehensive evaluation model on water source quality assessment is established and applied to main water source quality assessment in Fuxin City. The results show that fuzzy comprehensive evaluation model on drinking water source quality assessment not only could identify the source water quality level, but also could find out the main pollutants and main pollution sources. The final analysis results of different assessment methods indicate that fuzzy comprehensive evaluation model on drinking water source quality assessment possesses validity and favorable applicability.