This paper studies the CEA, CA199, IL-6 and MCP-1 expressions and their clinical significance in the serum of patients with pancreatic cancer. The concentrations of IL-6, MCP-1, CEA and CA199 were detected in 118 pancreatic cancer patients, 107 chronic pancreatitis patients and 113 healthy people. In addition, their individual and joint diagnostic values for pancreatic cancer were also evaluated. Compared with the normal control group, the CA199 and MCP-1 of the chronic pancreatitis group show significant difference. The CEA, CA199, IL-6 and MCP-1 of the pancreatic cancer group show significant increase. Compared with the chronic pancreatitis group, the CA199 and IL-6 of the pancreatic cancer group show significant increase. When the four indicators were used separately to differentiate the chronic pancreatitis group and pancreatic cancer group, the best diagnostic value is shown by the CA199. The diagnostic sensitivity and specificity are 72.30% and 74.90%. The IL-6 shows a sensitivity of 85.40% and a specificity of 57.50%. The joint diagnostic values of CA199 and IL-6 for differentiating chronic pancreatitis group and pancreatic cancer group are higher than any other indicator. The sensitivity and the specificity are 87.30% and 90.30%. The indicator shows a limited diagnostic value for clinical diagnosis. The joint indicators show a better diagnostic value than the individual indicator. It is a useful diagnostic method for helping the diagnosis of pancreatic cancer.