To observe the effects of Xipayi mouth rinse on the prostaglandin E₂ (PGE₂) production of Human Gingival Fibroblast (HGF) by stimulation of interleukin-1β (IL-1β), the HGF cells cultured by healthy human gingival tissue are used. With 1ng/mL IL-1β as a stimulating factor, HGF cells with 5×10⁴/mL cell density were inoculated into a culture plate with 24 holes, each containing 200μL cell suspension. 24h after incubation and adhering to the wall, the original culture liquid was abandoned and the mouth was washed 2 times after adding the drugs, which were grouped into control group (with 20m/L fetal bovine serum medium DMEM), IL-1β group (beta concentration is 1ng/mL), IL-1β+Xipayi mouth rinse group (end concentrations of were 50, 100, 200g/mL, respectively ), IL-1β+indomethacin (100ng/mL of endcentration) group. The enzyme immunoassay method is used to check the PGE₂ and observe the effects of different concentrations of Xipayi mouth rinse on PGE₂ levels in the HGF supernatant. Results show that Xipayi mouth rinse at different concentrations suppresses the PGE₂ production of HGF stimulated with 1ng/mL IL-1β significantly in a dose dependent manner. It is concluded that the human gingival fibroblast has the ability of synthesizing and secreting PGE₂, and IL-1β is a potent stimulation for HGF cell to generate PGE₂; and Xipayi mouth rinse can significantly inhibit the PGE₂ production of HGF by stimulation of IL-1β.