Analytical Method for Etheno DNA Adducts

  • TIAN Yongfeng;HOU Hongwei;LIU Yong;HU Qingyuan;WANG An
  • 1. China National Tobacco Quality Supervision and Test Center, Zhengzhou 450001, China ;2. Institute of Optics and Fine Mechanics, Chinese Academy of Science Anhui, Hefei 230031, China

Received date: 2011-11-14

  Revised date: 2012-05-10

  Online published: 2012-06-18


In order to deeply study etheno-DNA adducts, the variety of DNA oxidative damages, repair mechanisms, and their products are summarized. The generative mechanism in vivo and analytical method of etheno-DNA adducts, including 1, N6-ethenoadenine (εAde), 1, N2- ethenoguanine (εGua), and 3, N4-ethenocytosine (εCyt) are reviewed. The current analytical methods of etheno-DNA adducts have been discussed, which include Immunoaffinity Chromatography/32P-postlabelling technique (IC-32P), Gas Chromatogeraph-Mass Spectrometer (GC-MS). and Liquid Chromatography-tandem Mass Spectrometer (LC-MS/MS). IC-32P has the excellent performance on the sensibility, but when determining etheno-DNA adducts, complex operations and many steps have been involved. As analytical devices, the characteristics of mass spectrometer make the device obtain ideal sensibility and specificity. GC-MS method has the lower limit of determination than that for LC-MS/MS method. As sample derivatization is needed, the sample usually cost more for the method of GC-MS during the pre-handling; LC-MS/MS method offers many practical advantages on the pre-handing, stability, selectivity, and sensibility. These advantages could enhance the efficiency of samples analysis. LC-MS/MS is the ideal analytical method for the research on etheno-DNA adducts. The mechanism of DNA oxidative damage is inconclusive. As the biomarkers of DNA oxidative damages, etheno-DNA adducts possess significant meanings on the risk evaluation of lipid peroxidation.

Cite this article

TIAN Yongfeng;HOU Hongwei;LIU Yong;HU Qingyuan;WANG An . Analytical Method for Etheno DNA Adducts[J]. Science & Technology Review, 2012 , 30(17) : 73 -79 . DOI: 10.3981/j.issn.1000-7857.2012.17.012