In order to improve the social management on the human working fatigue, and reduce the economic loss due to the working fatigue, it is necessary to make a quantitative evaluation of the human working fatigue. First based on the combination of the entropy method, the factor analysis method and the comprehensive index method, four fatigue indexes are evaluated from the questionnaires and the experiments. Second, by using the improved fuzzy Borda method, the combination evaluation of the results is made for the above three comprehensive evaluation methods. Finally, the cluster analysis is made based on the results of the improved fuzzy Borda method. The enterprise could adjust its working tasks, incentive measures and working conditions in order to reduce the working fatigue.