This paper discusses the preparation of new porous titanium implants with different porosities and the influence of osteoblast cells on adhesion, proliferation, differentiation and osseointegration. The metal injection molding (MIM) is used to prepare the porous titanium implants with porosities of 30%, 40%, 50%, 60%, followed by the surface modification by means of acid-base heat treatment, and biomimetic deposition hydroxyapatite. With four groups of materials cultured with MG63, the influences of different porosities of the porous titanium implant on the adhesion, the proliferation and the differentiation of osteoblast cells MG63 in vitro are studied. With the four groups of materials implanted to the femoral of dogs for 12 weeks, the hard tissue slice and dye are made by using the Goldner's three color method, to study the effect of osseointegration. The results show that the 60% of cases of a group of materials are better than the other three groups on four aspects: the osteoblast quantity, the osteoblast stick form, the content of ALP and OCN and the formation of the bony combination. It is indicated that the high porosity implants after the surface modification with connected holes are propitious to the osteoblast cell's adhesion, the proliferation and the differentiation. They are also propitious to the osseointegration. This study provides a theoretical basis for the development of new porous titanium implants and implant dentures.